As armor coating, functionally graded (FG) tungsten/EUROFER multilayer was sprayed on EUROFER substrate for First Wall application in fusion field. Interface toughness between FG tungsten/EUROFER multilayer and EUROFER substrate was studied innovatively by a simple method based on the beam theory in this paper. To quantify interface toughness, the energy release rate was assessed by performing three and four-point bending tests on pre-cracked specimens at 550 degrees C and under high vacuum. The energy release rate during propagating of interfacial crack was determined to be 258 J/m(2) and 225 J/m(2) analytically and experimentally for samples with 3 and 5 layers as FG-layer, respectively, which were calculated based on multi bending tests. Cross-section and fracture microstructure show a vast of plasticity in FG-layer, particularly in FG-layer with a higher volume ratio of EUROFER. Interfacial fracture microstructure indicates interface adhesion consists of mechanical interlocking and metallurgical bonding.