Precise control of agarose media pore structure by regulating cooling rate | |
Chen, Chao1; Li, Xiunan1; Zhao, Dawei1; Li, Yaqiong2; Shi, Hong1; Ma, Guanghui1; Su, Zhiguo1,3 | |
刊名 | JOURNAL OF SEPARATION SCIENCE |
2017-11-01 | |
卷号 | 40期号:22页码:4467-4474 |
关键词 | Cooling Rate Low-field Nmr Spectroscopy Pore Size Pore Size Uniformity |
ISSN号 | 1615-9306 |
DOI | 10.1002/jssc.201700546 |
文献子类 | Article |
英文摘要 | A porous structure is the key factor to successful chromatography separation. Agarose gel as one of the most popular porous media has been extensively used in chromatography separation. As the cooling process in the agarose gelation procedure can directly influence the pore structure, ten kinds of 4% agarosemedia with different cooling rates from 0.132 to 16.7 degrees C/min were synthesized, and the pore structure was determined accurately by using low-field NMR spectroscopy. The curves of pore structure and cooling rate can be divided into two stages with the boundary of 6 degrees C/min. In stage I, the pore structure met a power equation with the decrease of the cooling rate, and in stage II, the process reached a plateau. Confirmatory experiments proved that, by adjusting the cooling rate, a precise control of the pore structure of agarose media can be realized, furthermore, cooling rate optimization was an effective way to control the pore size of agarose media and can further tailor the pore structure for more effective separation of different proteins. |
WOS关键词 | Size-exclusion Chromatography ; Stationary Phases ; Ionic-strength ; Gel Structure ; Transition ; Gradient ; Porosity ; Network |
WOS研究方向 | Chemistry |
语种 | 英语 |
WOS记录号 | WOS:000416228000019 |
资助机构 | National Natural Science Foundation of China(21376248 ; National Key Scientific Instrument and Equipment Development Project(2013YQ14040502) ; National Basic Research Program(2013CB733604) ; 21506228) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/23514] |
专题 | 过程工程研究所_研究所(批量导入) |
通讯作者 | Li, Xiunan; Su, Zhiguo |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 2.Sichuan Univ, Key Lab Drug Targeting & Drug Delivery Syst, Minist Educ, West China Sch Pharm, Chengdu, Sichuan, Peoples R China 3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Chao,Li, Xiunan,Zhao, Dawei,et al. Precise control of agarose media pore structure by regulating cooling rate[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(22):4467-4474. |
APA | Chen, Chao.,Li, Xiunan.,Zhao, Dawei.,Li, Yaqiong.,Shi, Hong.,...&Su, Zhiguo.(2017).Precise control of agarose media pore structure by regulating cooling rate.JOURNAL OF SEPARATION SCIENCE,40(22),4467-4474. |
MLA | Chen, Chao,et al."Precise control of agarose media pore structure by regulating cooling rate".JOURNAL OF SEPARATION SCIENCE 40.22(2017):4467-4474. |
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