Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors

doi:10.1080/02705060.2012.657828

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	Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors
	Wu, Li 2; Feng, Weisong 1; Feng, WS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.
刊名	JOURNAL OF FRESHWATER ECOLOGY
	2012-06-01
卷号	27 期号:2 页码:229-241
关键词	Fingerprinting Community Structure Zooplankton Rapd Pcr-dgge Lake Dianchi
ISSN号	0270-5060
DOI	10.1080/02705060.2012.657828
文献子类	Article
英文摘要	The plankton community in Fubao Bay of Lake Dianchi, a large, shallow and highly polluted freshwater lake in China, was investigated on three dates. First, a total of 76 zooplankton species were identified by morphological characteristics of which 44 were protozoa, 20 rotifer, and 12 crustacean. Then, the plankton community was characterized by using random amplified polymorphic DNA (RAPD) and Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) over the time period. Morphological composition, RAPD fingerprints, PCR-DGGE fingerprints, and unweighted pair-group method using arithmetic averages clustering analysis all showed that samples within a season were generally clustered into the same group. The results also revealed that RAPD fingerprints and PCR-DGGE fingerprints were identical to morphological composition in characterizing community similarity. Canonical correspondence analysis based on morphological composition, RAPD fingerprints, and PCR-DGGE fingerprints all suggested that the factors strongly correlated with the first two ordination axes were seasonally different, further suggesting high- temporal heterogeneity in Lake Dianchi. There were close relationships between morphological composition, RAPD and PCR-DGGE fingerprints, and: (1) total nitrogen in summer, (2) total phosphorus in autumn, and (3) BOD5 or NO2-N in winter. Results of this study suggest that molecular techniques are acceptable methods for examining plankton community composition and measuring plankton community responses to stress and perturbation.; The plankton community in Fubao Bay of Lake Dianchi, a large, shallow and highly polluted freshwater lake in China, was investigated on three dates. First, a total of 76 zooplankton species were identified by morphological characteristics of which 44 were protozoa, 20 rotifer, and 12 crustacean. Then, the plankton community was characterized by using random amplified polymorphic DNA (RAPD) and Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) over the time period. Morphological composition, RAPD fingerprints, PCR-DGGE fingerprints, and unweighted pair-group method using arithmetic averages clustering analysis all showed that samples within a season were generally clustered into the same group. The results also revealed that RAPD fingerprints and PCR-DGGE fingerprints were identical to morphological composition in characterizing community similarity. Canonical correspondence analysis based on morphological composition, RAPD fingerprints, and PCR-DGGE fingerprints all suggested that the factors strongly correlated with the first two ordination axes were seasonally different, further suggesting high- temporal heterogeneity in Lake Dianchi. There were close relationships between morphological composition, RAPD and PCR-DGGE fingerprints, and: (1) total nitrogen in summer, (2) total phosphorus in autumn, and (3) BOD5 or NO2-N in winter. Results of this study suggest that molecular techniques are acceptable methods for examining plankton community composition and measuring plankton community responses to stress and perturbation.
WOS关键词	GRADIENT GEL-ELECTROPHORESIS ; GENETIC DIVERSITY ; TROPHIC STATUS ; WATER-QUALITY ; ECOSYSTEMS ; MICROORGANISMS ; POPULATIONS ; INDICATORS ; ECOLOGY ; DONGHU
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000304454400006
资助机构	Major State Basic Research Development Program of China[2008CB418105]; Anhui Colleges[KJ2011Z308] ; Major State Basic Research Development Program of China[2008CB418105]; Anhui Colleges[KJ2011Z308] ; Major State Basic Research Development Program of China[2008CB418105]; Anhui Colleges[KJ2011Z308] ; Major State Basic Research Development Program of China[2008CB418105]; Anhui Colleges[KJ2011Z308]
公开日期	2012-09-25
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16855]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
通讯作者	Feng, WS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China 2.Hefei Normal Univ, Dept Life Sci, Hefei 230061, Peoples R China
推荐引用方式 GB/T 7714	Wu, Li,Feng, Weisong,Feng, WS . Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors[J]. JOURNAL OF FRESHWATER ECOLOGY,2012,27(2):229-241.
APA	Wu, Li,Feng, Weisong,&Feng, WS .(2012).Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors.JOURNAL OF FRESHWATER ECOLOGY,27(2),229-241.
MLA	Wu, Li,et al."Temporal heterogeneity of plankton community in Lake Dianchi and its relation to environmental factors".JOURNAL OF FRESHWATER ECOLOGY 27.2(2012):229-241.