Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth

doi:10.1016/j.snb.2017.08.088

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	Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth
	Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu; Wei Du
刊名	Sensors and Actuators B-Chemical
	4296
卷号	255 页码:735-744
DOI	10.1016/j.snb.2017.08.088
产权排序	2
文献子类	Article
英文摘要	C. elegans is a leading model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradient-customizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 108 cells mL-1 and further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen.
WOS关键词	CAENORHABDITIS-ELEGANS ; MICROFLUIDIC DEVICE ; QUANTITATIVE-ANALYSIS ; DEVELOPMENTAL-STAGES ; LIFE-SPAN ; GENETICS ; PLATFORM ; CHEMOTAXIS ; LONGEVITY ; PATHWAYS
WOS研究方向	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000414151800088
资助机构	National Natural Science Foundation of China ; National Key R&D Program of China
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9869]
专题	青岛生物能源与过程研究所_单细胞中心
通讯作者	Wei Du
推荐引用方式 GB/T 7714	Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,Wei Du. Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth[J]. Sensors and Actuators B-Chemical,4296,255:735-744.
APA	Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,&Wei Du.(4296).Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth.Sensors and Actuators B-Chemical,255,735-744.
MLA	Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,et al."Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth".Sensors and Actuators B-Chemical 255(4296):735-744.