Unusual acylation of chloramphenicol in Lysobacter enzymogenes , a biocontrol agent with intrinsic resistance to multiple antibiotics

doi:DOI 10.1186/s12896-017-0377-y

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 酶工程团队

	Unusual acylation of chloramphenicol in Lysobacter enzymogenes , a biocontrol agent with intrinsic resistance to multiple antibiotics
	Wei Zhang; Justin Huffman; Shengying Li; Yuemao Shen; Liangcheng Du
刊名	BMC BIOTECHNOLOGY
	2017
卷号	17 页码:59-67
DOI	DOI 10.1186/s12896-017-0377-y
文献子类	Article
英文摘要	Background: The environmental gliding bacteria Lysobacter are emerging as a new group of bio control agents dueto their prolific production of lytic enzymes and potent antibiotic natural products. These bacteria are intrinsicallyresistant to many antibioti cs, but the mechanisms behind the antibiotic resistance have not been investigated. Results: Previously, we have used chloramphenicol acetyl transferase gene (ca t ) as a selection marker in genetic manipulation of natural product biosynthetic genes in Lysobac ter , because chloram phenicol is one of the two common antibiotics that Lysobacter are susceptible to. Here, we found L. enzymogenes, the most studied species of this genus, could still grow in th e p resen c e of a lo w c on centratio n of chlo ramphen icol . Th ree c hl oramphe nicol derivatives (1–3) with an unusual acylation pattern were identified in a cat-containing mutant of L. enzymogenes and in the wild type. The compounds included chloramphenicol 3'-isobutyrate (1), a new compound chloramphenicol 1'-isobutyrate (2), and a rare chloramphenicol 3'-isovalerate (3). Furthermore, a mutation of a global regulator gene (clp) or a Gcn5-related N acetyltransferase (GNAT) gene in L. enzymogenes led to nearly no growth in media containing chloramphenicol, whereas a complementation of clp restored the chloramphenicol acylation as well as antibiotic HSAFproduction in the clp mutant. Conclusions: The results indicated that L. enzymogenes contains a pool of unusual acyl donors for enzymatic modification of chloramphenicol that confers the resistance, which may involve the Clp-GNAT regulatory system.Because Lysobacter are ubiquitous inhabitants of soil and water, the finding may have important implications in understanding microbial competitions and bioactive natural product regulation.
WOS关键词	Lysobacter ; Antibiotic resistance ; Chloramphenicol ; Acylation
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000405335600002
资助机构	NSFC (31329005 and 31570 030) ; Nebraska Research Initiatives
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9857]
专题	青岛生物能源与过程研究所_酶工程团队
推荐引用方式 GB/T 7714	Wei Zhang,Justin Huffman,Shengying Li,et al. Unusual acylation of chloramphenicol in Lysobacter enzymogenes , a biocontrol agent with intrinsic resistance to multiple antibiotics[J]. BMC BIOTECHNOLOGY,2017,17:59-67.
APA	Wei Zhang,Justin Huffman,Shengying Li,Yuemao Shen,&Liangcheng Du.(2017).Unusual acylation of chloramphenicol in Lysobacter enzymogenes , a biocontrol agent with intrinsic resistance to multiple antibiotics.BMC BIOTECHNOLOGY,17,59-67.
MLA	Wei Zhang,et al."Unusual acylation of chloramphenicol in Lysobacter enzymogenes , a biocontrol agent with intrinsic resistance to multiple antibiotics".BMC BIOTECHNOLOGY 17(2017):59-67.