A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency

doi:10.1016/j.snb.2018.01.077

	A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency
	Wang, Xixian 1,2,3; Ge, Anle 1; Hu, Liang 1; Feng, Xiaojun 1; Du, Wei 1; Liu, Bi-Feng 1
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018-05-01
卷号	260 页码:311-319
关键词	Microfluidic Chip C. Elegans Size-dependent Sorting Electrotaxis Microfilters
ISSN号	0925-4005
DOI	10.1016/j.snb.2018.01.077
文献子类	Article
英文摘要	C. elegans is a leading model organism for addressing many fundamental biology issues. Sufficient quantities of the worms with specific developmental stage are needed in C. elegans studies due to the size-dependent features shown in C. elegans. In this work, we presented a simple and feasible microfluidic chip to realize simultaneous sorting of C. elegans with different sizes by graduated microfilters. The micro-device was fabricated with PDMS-agarose hybrid layers consisting of 5 arrays of geometrically optimized square columns as microfilters for separating worms of different sizes. Fluid flow and electric field were simultaneously applied to drive the worms to rapidly and directionally pass through the microfilters, resulting in increased sorting efficiency and accuracy. Further, the sorted worms could be easily collected from 4 grooves of the agarose layer by slipping the PDMS layer to the top of grooves. With this sorting strategy, we demonstrated the feasibility of simultaneously sorting different sizes of C. elegans with high efficiency and accuracy (>95%) and throughput of hundreds of worms per minute. Therefore, the micro-device could be a promising tool to C. elegans assays that need a large number of stage-synchronized worms. It could also be useful for body size-based mutant screening and taxis-response studies in C. elegans. (c) 2018 Elsevier B.V. All rights reserved.
WOS关键词	NEMATODE CAENORHABDITIS-ELEGANS ; HIGH-THROUGHPUT ; IN-VIVO ; NEURONAL-ACTIVITY ; SYSTEM ; DEVICE ; PLATFORM ; MOTILITY ; SCREENS
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000424884300037
资助机构	National Key R&D Program of China(2016YFF0100801) ; National Natural Science Foundation of China(21475049 ; Fundamental Research Funds for the Central Universities(2016YXZD061) ; NIH National Centre for Research Resources ; 31471257 ; 21275060)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10747]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Du, Wei; Liu, Bi-Feng
作者单位	1.Huazhong Univ Sci & Technol, Coll Life Sci & Technol,Key Lab Biomed Photon, Dept Biomed Engn,Wuhan Natl Lab Optoelect,MOE, Syst Biol Theme,Hubei Bioinformat & Mol Imaging K, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xixian,Ge, Anle,Hu, Liang,et al. A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,260:311-319.
APA	Wang, Xixian,Ge, Anle,Hu, Liang,Feng, Xiaojun,Du, Wei,&Liu, Bi-Feng.(2018).A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency.SENSORS AND ACTUATORS B-CHEMICAL,260,311-319.
MLA	Wang, Xixian,et al."A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency".SENSORS AND ACTUATORS B-CHEMICAL 260(2018):311-319.