Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans

doi:10.1080/10826068.2017.1350979

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans
	Chen, Guoqiang 1,2; Wang, Jiming 1; Su, Yulong 3; Zhu, Youshuang 4; Zhang, Ge 1,3; Zhao, Hongwei 1; Liu, Haobao 3; Yang, Ying 1; Nian, Rui 1; Zhang, Haibo 1
刊名	PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
	2017
卷号	47 期号:10 页码:963-969
关键词	Aureobasidium Pullulans Fermentation Conditions Ftir Nmr Pullulan Tlc
ISSN号	1082-6068
DOI	10.1080/10826068.2017.1350979
文献子类	Article
英文摘要	Pullulan with different molecular-weight could be applied in various fields. A UV-induced mutagenesis Aureobasidium pullulans UVMU6-1 was obtained from the strain A. pullulans CGMCC3.933 for the production of low-molecular-weight pullulan. First, the obtained polysaccharide from A. pullulans UVMU6-1 was purified and identified to be pullulan with thin-layer chromatography, Fourier transform infrared, and nuclear magnetic resonance. Then, culture medium and conditions for this strain were optimized by flask fermentation. Based on the optimized medium and culture conditions (pH 4, addition of 4g/L Tween 80 for 96hr of cultivation), continuously fermentation was performed. The highest pullulan production and dry biomass was 109 and 125g/L after fermentation for 114hr, respectively. The average productivity was about 1g/L/hr, which was intensively higher than the previous reported. This study would lay foundations for the industrial production of pullulan.
WOS关键词	MOLECULAR-WEIGHT PULLULAN ; SUGAR METABOLISM ; TWEEN 80 ; FERMENTATION ; STRAIN ; OPTIMIZATION ; POTATO ; LIPASE ; EXOPOLYSACCHARIDES ; POLYSACCHARIDE
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000418064000003
资助机构	National Natural Science Foundation of China (NSF)(31400084) ; Natural Science Foundation of Shandong Province(ZR2014CQ008) ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; Youth Innovation Promotion Association (CAS)(2017252)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10521]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
通讯作者	Zhang, Haibo; Wei, Yuxi; Xian, Mo
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao, Peoples R China 4.Jining Med Univ, Sch Biol Sci, Jining, Peoples R China 5.189 Songling Rd, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Chen, Guoqiang,Wang, Jiming,Su, Yulong,et al. Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2017,47(10):963-969.
APA	Chen, Guoqiang.,Wang, Jiming.,Su, Yulong.,Zhu, Youshuang.,Zhang, Ge.,...&Xian, Mo.(2017).Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,47(10),963-969.
MLA	Chen, Guoqiang,et al."Pullulan production from synthetic medium by a new mutant of Aureobasidium pullulans".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 47.10(2017):963-969.