Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers | |
Xie, Huayong1,2; Zhao, Yongxiang1,2; Wang, Jing1,2; Zhang, Zhengfeng2; Yang, Jun2 | |
刊名 | BIOMOLECULAR NMR ASSIGNMENTS |
2018-10-01 | |
卷号 | 12期号:2页码:323-328 |
关键词 | Aquaporin Z Solid-state NMR Resonance assignment Reverse labeling Secondary structure |
ISSN号 | 1874-2718 |
DOI | 10.1007/s12104-018-9832-5 |
英文摘要 | Aquaporin Z is the first identified prokaryotic water channel in Escherichia coli with a high water permeability and strict substrate selectivity. Here we report nearly complete (94% of amino acid residues) C-13 and N-15 chemical shift assignments of AqpZ reconstituted in the lipid bilayers using a set of 2D and 3D magic angle spinning solid-state NMR spectra. Secondary structure of AqpZ predicted from chemical shift assignments is generally similar to that of X-ray structure with a number of differences in loop and near-loop regions. The BMRB accession number of the assignments is 27244. |
资助项目 | National Key R&D Program of China[2016YFA0501200] ; National Key R&D Program of China[2017YFA0505400] ; National Natural Science Foundation of China[31770798] ; National Natural Science Foundation of China[21425523] ; National Natural Science Foundation of China[21405171] ; National Natural Science Foundation of China[21775161] |
WOS关键词 | PROTEIN SECONDARY STRUCTURE ; X-RAY-STRUCTURE ; ESCHERICHIA-COLI ; WATER PERMEATION ; CHANNEL ; AQPZ ; SELECTIVITY |
WOS研究方向 | Biophysics ; Spectroscopy |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000443824500020 |
资助机构 | National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
内容类型 | 期刊论文 |
源URL | [http://ir.wipm.ac.cn/handle/112942/13088] |
专题 | 中国科学院武汉物理与数学研究所 |
通讯作者 | Yang, Jun |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Xie, Huayong,Zhao, Yongxiang,Wang, Jing,et al. Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers[J]. BIOMOLECULAR NMR ASSIGNMENTS,2018,12(2):323-328. |
APA | Xie, Huayong,Zhao, Yongxiang,Wang, Jing,Zhang, Zhengfeng,&Yang, Jun.(2018).Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers.BIOMOLECULAR NMR ASSIGNMENTS,12(2),323-328. |
MLA | Xie, Huayong,et al."Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers".BIOMOLECULAR NMR ASSIGNMENTS 12.2(2018):323-328. |
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