Dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants

doi:10.1111/pbi.12721

	Dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants
	Liu, Caixiang 2; Du, Ba 3; Hao, Fuhua 2; Lei, Hehua 2; Wan, Qianfen 1,2,4; He, Guangcun 3; Wang, Yulan 2,5; Tang, Huiru 1,2,4
刊名	PLANT BIOTECHNOLOGY JOURNAL
	2017-10-01
卷号	15 期号:10 页码:1346-1357
关键词	Brown Planthopper Resistant And Susceptible Rice Metabonomics Real-time Pcr Gc-fid/ms
ISSN号	1467-7644
DOI	10.1111/pbi.12721
文献子类	Article
英文摘要	Brown planthopper (Nilaparvata lugens Stal, BPH) causes huge economic losses in rice-growing regions, and new strategies for combating BPH are required. To understand how BPHs respond towards BPH-resistant plants, we systematically analysed the metabolic differences between BPHs feeding on the resistant and susceptible plants using NMR and GC-FID/MS. We also measured the expression of some related genes involving glycolysis and biosyntheses of trehalose, amino acids, chitin and fatty acids using real-time PCR. BPH metabonome was dominated by more than 60 metabolites including fatty acids, amino acids, carbohydrates, nucleosides/nucleotides and TCA cycle intermediates. After initial 12 h, BPHs feeding on the resistant plants had lower levels of amino acids, glucose, fatty acids and TCA cycle intermediates than on the susceptible ones. The levels of these metabolites recovered after 24 h feeding. This accompanied with increased level in trehalose, choline metabolites and nucleosides/nucleotides compared with BPH feeding on the susceptible plants. Decreased levels of BPH metabolites at the early feeding probably resulted from less BPH uptakes of sap from resistant plants and recovery of BPH metabolites at the later stage probably resulted from their adaptation to the adverse environment with their increased hopping frequency to ingest more sap together with contributions from yeast-like symbionts in BPHs. Throughout 96 h, BPH feeding on the resistant plants showed significant up-regulation of chitin synthase catalysing biosynthesis of chitin for insect exoskeleton, peritrophic membrane lining gut and tracheae. These findings provided useful metabolic information for understanding the BPH-rice interactions and perhaps for developing new BPH-combating strategies.
WOS关键词	NILAPARVATA-LUGENS STAL ; PROTEOMIC ANALYSIS ; ARGININE KINASE ; GENOTYPES ; IDENTIFICATION ; MECHANISM ; FECUNDITY ; INSECTS ; NMR ; METABONOMICS
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000423463200013
资助机构	National Natural Science Foundation of China(31200208) ; National Natural Science Foundation of China(31200208) ; National Natural Science Foundation of China(31200208) ; National Natural Science Foundation of China(31200208) ; National Natural Science Foundation of China(31200208) ; National Natural Science Foundation of China(31200208) ; National Natural Science Foundation of China(31200208) ; National Natural Science Foundation of China(31200208)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12844]
专题	中国科学院武汉物理与数学研究所
通讯作者	Wang, Yulan; Tang, Huiru
作者单位	1.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Metabon & Syst Biol Lab, Sch Life Sci,Shanghai Int Ctr Mol Phen, Shanghai, Peoples R China 2.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, CAS Key Lab Magnet Resonance Biol Syst, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan, Hubei, Peoples R China 3.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan, Hubei, Peoples R China 4.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Metabon & Syst Biol Lab,Zhongshan Hosp, Shanghai Int Ctr Mol Phen,State Key Lab Genet En, Shanghai, Peoples R China 5.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Caixiang,Du, Ba,Hao, Fuhua,et al. Dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants[J]. PLANT BIOTECHNOLOGY JOURNAL,2017,15(10):1346-1357.
APA	Liu, Caixiang.,Du, Ba.,Hao, Fuhua.,Lei, Hehua.,Wan, Qianfen.,...&Tang, Huiru.(2017).Dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants.PLANT BIOTECHNOLOGY JOURNAL,15(10),1346-1357.
MLA	Liu, Caixiang,et al."Dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants".PLANT BIOTECHNOLOGY JOURNAL 15.10(2017):1346-1357.