DNA quadruplexes as molecular scaffolds for controlled assembly of fluorogens with aggregation-induced emission

doi:10.1039/c8sc00001h

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	DNA quadruplexes as molecular scaffolds for controlled assembly of fluorogens with aggregation-induced emission
	Zhou, Jun 1; Xu, Guohua 2; Li, Conggang 2; Ling, Pinghua 1; Liu, Bin 3; Ju, Huangxian 1; Lei, Jianping 1; Zhu, Longyi 1
刊名	CHEMICAL SCIENCE
	2018-03-07
卷号	9 期号:9 页码:2559-2566
DOI	10.1039/c8sc00001h
文献子类	Article
英文摘要	Aggregation-induced emission (AIE) can be generated due to the restriction of intramolecular motions. The controllable assembly of fluorogens with AIE properties (AIEgens) is able to provide a new opportunity for precise manipulation of fluorescent signal transduction. Here, a tetrapod DNA quadruplex (TP-G4) was designed as a molecular scaffold for assembly and precise modulation of light emission of an oligonucleotide-grafted fluorogen with aggregation-induced emission (Oligo-AIEgen). The Oligo-AIEgen was synthesized by attaching the AIEgen to the 3'-terminus of the oligonucleotide through a dibenzylcyclooctyne mediated coupling reaction. The AIEgen emitted no detectable fluorescence in the context of a double-stranded structure. When hybridized to the parallel-stranded TP-G4, several AIEgens were located in close proximity to generate fluorescence. The fluorescence intensity has been precisely regulated by manipulation of the spacer length between the core structure of the scaffold and AIEgen, as well as by altering the quartet number of the G-quadruplex. Similar control of fluorescence was also demonstrated using tetramolecular and bimolecular i-motif quadruplex structures as the scaffolds. These scaffolds provide a proof of concept on the manipulation of molecular interactions, which forms a universal molecular tool for the design of new biosensing strategies.
WOS关键词	TURN-ON FLUORESCENCE ; LIGHT-UP BIOPROBE ; C-MYC PROMOTER ; I-MOTIF ; CANCER-CELLS ; ACID ; OLIGONUCLEOTIDE ; PROBE ; TETRAPHENYLETHENE ; PHOSPHORESCENCE
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000431087700021
资助机构	National Key Technologies RD Program(2016YFC0302500) ; National Key Technologies RD Program(2016YFC0302500) ; National Natural Science Foundation of China(21375060 ; National Natural Science Foundation of China(21375060 ; Singapore National Foundation(R279-000-444-281 ; Singapore National Foundation(R279-000-444-281 ; 21635005 ; 21635005 ; R279-000-483-281) ; R279-000-483-281) ; 21675084) ; 21675084) ; National Key Technologies RD Program(2016YFC0302500) ; National Key Technologies RD Program(2016YFC0302500) ; National Natural Science Foundation of China(21375060 ; National Natural Science Foundation of China(21375060 ; Singapore National Foundation(R279-000-444-281 ; Singapore National Foundation(R279-000-444-281 ; 21635005 ; 21635005 ; R279-000-483-281) ; R279-000-483-281) ; 21675084) ; 21675084)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12054]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 3.Natl Univ Singapore, Dept Chem & Biomol Engn, 4 Sci Dr 4, Singapore 117585, Singapore
推荐引用方式 GB/T 7714	Zhou, Jun,Xu, Guohua,Li, Conggang,et al. DNA quadruplexes as molecular scaffolds for controlled assembly of fluorogens with aggregation-induced emission[J]. CHEMICAL SCIENCE,2018,9(9):2559-2566.
APA	Zhou, Jun.,Xu, Guohua.,Li, Conggang.,Ling, Pinghua.,Liu, Bin.,...&Zhu, Longyi.(2018).DNA quadruplexes as molecular scaffolds for controlled assembly of fluorogens with aggregation-induced emission.CHEMICAL SCIENCE,9(9),2559-2566.
MLA	Zhou, Jun,et al."DNA quadruplexes as molecular scaffolds for controlled assembly of fluorogens with aggregation-induced emission".CHEMICAL SCIENCE 9.9(2018):2559-2566.