Ultrasensitive MicroRNA Assay via Surface Plasmon Resonance Responses of Au@Ag Nanorods Etching

doi:10.1021/acs.analchem.7b02920

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Ultrasensitive MicroRNA Assay via Surface Plasmon Resonance Responses of Au@Ag Nanorods Etching
	Zhang, Ting-Ting 1,2; Chen, Hong-Yuan 1,2; Liu, Mali 3; Xu, Jing-Juan 1,2; Zhao, Wei 1,2; Song, Juan 1,2; Gu, Yu 1,2; Li, Mei-Xing 1,2
刊名	ANALYTICAL CHEMISTRY
	2017-10-03
卷号	89 期号:19 页码:10585-10591
DOI	10.1021/acs.analchem.7b02920
文献子类	Article
英文摘要	Quantification of trace serum circulate microRNAs is extremely important in clinical diagnosis but remains a great challenge. Herein we developed an ultrasensitive platform for microRNA 141 (miR-141) detection based on a silver coated gold nanorods (Au@Ag NRs) etching process accompanied by surface plasmon resonance (SPR) shift. Both SPR absorption and scattering responses were monitored. Combined amplification cascades of catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) with the sensitive SPR responses of plasmonic Au@Ag NRs, the proposed bioassay exhibited ultrahigh sensitivity toward miRNA-141 with dynamic range from 5.0 X 10(-17) M to 1.0 x 10(-11) M. With target concentration higher than 1.0 X 10(-13) M, the color of the solution changed obviously that could be observed with naked eyes. Under dark-field microscopy observation of individual particle, a limit of detection down to 50 aM could be achieved. Owing to the superior sensitivity and selectivity, the proposed method was applied to detecting trace microRNA in serum. Similar SPR assays could be developed simply by redesigning the switching aptamer for the detections of other microRNAs or targets such as small molecule, DNA, or protein. Considering the convenient operation, good performance and simple observation modes of this method, it may have great potential in trace bioanalysis for clinical applications.
WOS关键词	G-QUADRUPLEX/HEMIN DNAZYME ; ELECTROCHEMILUMINESCENT BIOSENSOR ; ENERGY-TRANSFER ; GOLD NANORODS ; CANCER ; NANOPARTICLES ; PLATFORM ; THERAPY ; MIRNAS
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000412716800064
资助机构	Science and Technology Ministry of China(2016YFA0201200) ; Science and Technology Ministry of China(2016YFA0201200) ; National Natural Science Foundation of China(21327902 ; National Natural Science Foundation of China(21327902 ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Innovation Cross Team of Chinese Academy of Sciences ; Innovation Cross Team of Chinese Academy of Sciences ; 21535003) ; 21535003) ; Science and Technology Ministry of China(2016YFA0201200) ; Science and Technology Ministry of China(2016YFA0201200) ; National Natural Science Foundation of China(21327902 ; National Natural Science Foundation of China(21327902 ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; Innovation Cross Team of Chinese Academy of Sciences ; Innovation Cross Team of Chinese Academy of Sciences ; 21535003) ; 21535003)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11423]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China 2.Nanjing Univ, Sch Chem & Chem Engn, Collaborat Innovat Ctr Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ting-Ting,Chen, Hong-Yuan,Liu, Mali,et al. Ultrasensitive MicroRNA Assay via Surface Plasmon Resonance Responses of Au@Ag Nanorods Etching[J]. ANALYTICAL CHEMISTRY,2017,89(19):10585-10591.
APA	Zhang, Ting-Ting.,Chen, Hong-Yuan.,Liu, Mali.,Xu, Jing-Juan.,Zhao, Wei.,...&Li, Mei-Xing.(2017).Ultrasensitive MicroRNA Assay via Surface Plasmon Resonance Responses of Au@Ag Nanorods Etching.ANALYTICAL CHEMISTRY,89(19),10585-10591.
MLA	Zhang, Ting-Ting,et al."Ultrasensitive MicroRNA Assay via Surface Plasmon Resonance Responses of Au@Ag Nanorods Etching".ANALYTICAL CHEMISTRY 89.19(2017):10585-10591.