WS2 and MoS2 biosensing platforms using peptides as probe biomolecules

doi:10.1038/s41598-017-10221-4

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	WS2 and MoS2 biosensing platforms using peptides as probe biomolecules
	Fu, Caihong 2; Fan, Jun 2; Sun, Xiuxia 2; Xiao, Jianxi 1,2; Wang, Jie 1; Zhao, Sha 2; Yao, Linyan 2
刊名	SCIENTIFIC REPORTS
	2017-08-31
卷号	7
DOI	10.1038/s41598-017-10221-4
文献子类	Article
英文摘要	Biosensors based on the two-dimensional layered nanomaterials transition metal dichalcogenides such as WS2 and MoS2 have shown broad applications, while they largely rely on the utilization of single stranded DNA as probe biomolecules. Herein we have constructed novel WS2- and MoS2-based biosensing platforms using peptides as probe biomolecules. We have revealed for the first time that the WS2 and MoS2 nanosheets display a distinct adsorption for Arg amino acid and particularly, Arg-rich peptdies. We have demonstrated that the WS2 and MoS2 dramatically quench the fluorescence of our constructed Arg-rich probe peptide, while the hybridization of the probe peptide with its target collagen sequence leads to the fluorescence recovery. The WS2-based platform provides a sensitive fluorescence-enhanced assay that is highly specific to the target collagen peptide with little interferences from other proteins. This assay can be applied for quantitative detection of collagen biomarkers in complex biological fluids. The successful development of WS2- and MoS2-based biosensors using non-ssDNA probes opens great opportunities for the construction of novel multifunctional biosensing platforms, which may have great potential in a wide range of biomedical field.
WOS关键词	GRAPHENE OXIDE ; FLUORESCENT BIOSENSOR ; MONOCLONAL-ANTIBODIES ; POLYMER NANOCOMPOSITES ; SENSING APPLICATIONS ; CARBON NANOTUBES ; TRIPLE-HELIX ; II COLLAGEN ; NANOSHEETS ; DNA
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000408781200149
资助机构	National Natural Science Foundation of China(21305056) ; National Natural Science Foundation of China(21305056) ; Fundamental Research Funds for the Central Universities(lzujbky-2016-k10) ; Fundamental Research Funds for the Central Universities(lzujbky-2016-k10) ; open fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; open fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; National Natural Science Foundation of China(21305056) ; National Natural Science Foundation of China(21305056) ; Fundamental Research Funds for the Central Universities(lzujbky-2016-k10) ; Fundamental Research Funds for the Central Universities(lzujbky-2016-k10) ; open fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402) ; open fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11352]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Fu, Caihong,Fan, Jun,Sun, Xiuxia,et al. WS2 and MoS2 biosensing platforms using peptides as probe biomolecules[J]. SCIENTIFIC REPORTS,2017,7.
APA	Fu, Caihong.,Fan, Jun.,Sun, Xiuxia.,Xiao, Jianxi.,Wang, Jie.,...&Yao, Linyan.(2017).WS2 and MoS2 biosensing platforms using peptides as probe biomolecules.SCIENTIFIC REPORTS,7.
MLA	Fu, Caihong,et al."WS2 and MoS2 biosensing platforms using peptides as probe biomolecules".SCIENTIFIC REPORTS 7(2017).