Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease

doi:10.1007/s12010-016-2260-z

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease
	Chen, Yanke 1; Xie, Hao 2; Yang, Jun 1; Li, Qichang 2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2017-03-01
卷号	181 期号:3 页码:939-947
关键词	Tagremoval Stericocclusions Maltosebinding Protein Integral Membrane Protein Expression And Purification Tev Protease
DOI	10.1007/s12010-016-2260-z
文献子类	Article
英文摘要	Tag removal is a prerequisite issue for structural and functional analysis of affinity-purified membrane proteins. The present study took a MBP-fused membrane protein, MrpF, as a model to investigate the tag removal by TEV protease. Influences of the linking sequence between TEV cleavage site and MrpF on protein expression and predicted secondary structure were investigated. The steric accessibility of TEV protease to cleavage site of MBP-fused MrpF was explored. It was found that reducing the size of hydrophilic group of detergents and/or extending the linking sequence between cleavage site and target protein can significantly improve the accessibility of the cleavage site and promote tag removal by TEV protease.
WOS关键词	ETCH VIRUS PROTEASE ; ESCHERICHIA-COLI ; AFFINITY TAGS ; DETERGENT SOLUTIONS ; BACILLUS-SUBTILIS ; PURIFICATION ; EXPRESSION ; STRATEGIES ; TOPOLOGY ; GENOMES
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000394992900006
资助机构	Ministry of Science and Technology(2016YFA0501200) ; Ministry of Science and Technology(2016YFA0501200) ; National Natural Science Foundation of China(31500603 ; National Natural Science Foundation of China(31500603 ; Fundamental Research Funds for the Central Universities(WUT 2016IB006) ; Fundamental Research Funds for the Central Universities(WUT 2016IB006) ; 21425523) ; 21425523) ; Ministry of Science and Technology(2016YFA0501200) ; Ministry of Science and Technology(2016YFA0501200) ; National Natural Science Foundation of China(31500603 ; National Natural Science Foundation of China(31500603 ; Fundamental Research Funds for the Central Universities(WUT 2016IB006) ; Fundamental Research Funds for the Central Universities(WUT 2016IB006) ; 21425523) ; 21425523)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/10888]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yanke,Xie, Hao,Yang, Jun,et al. Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,181(3):939-947.
APA	Chen, Yanke,Xie, Hao,Yang, Jun,&Li, Qichang.(2017).Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,181(3),939-947.
MLA	Chen, Yanke,et al."Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 181.3(2017):939-947.