Interactions of Vitamin K-3 with Herring-Sperm DNA Using Spectroscopy and Electrochemistry

	Interactions of Vitamin K-3 with Herring-Sperm DNA Using Spectroscopy and Electrochemistry
	Huang, Jianhang; Wang, Xingming; Fei, Dan; Ding, Lisheng; Wang, XM, SW Univ Sci & Technol, Sch Mat Sci & Engn, Mianyang 621010, Peoples R China
刊名	APPLIED SPECTROSCOPY
	2010
卷号	64 期号:10 页码:1126-1130
关键词	Uv-vis Spectroscopy Vitamin K-3 Herring-sperm Dna Dna-drug Interaction
ISSN号	0003-7028
产权排序	2
英文摘要	By means of ultraviolet visible (UV-Vis) and fluorescence spectra, the binding ratio between vitamin K-3 and herring-sperm DNA in a physiological pH environment (pH = 7.40) was determined as n(K3): n(DNA) = 2: 1, and the binding constants of vitamin K-3 binding to DNA at different temperatures were determined as K-298K(theta) = 1.28 x 10(5) L.mol(-1) and K-310K(theta) =7.19 x 10(4) L.mol(-1), which were confirmed using the double reciprocal method are Delta H-r(m)theta) = -3.57 x 10(4) J.mol(-1), Delta(r)G(m)(theta)) = -2.92 x 10(4) J.mol(-1), and Delta S-r(m)theta = 217.67.J.mol(-1)K(-1). The driving power of this process was enthalpy. An intercalation binding of the vitamin K-3 with DNA was supported by a competitive experiment using acridine orange (AO) as a spectral probe. By combination analysis of the Scatchard method and cyclic voltammetry, we suggested that the interaction mode between vitamin K-3 and herring-sperm DNA would be a mixed mode. The quinonoid, duality fused-ring of vitamin K-3 can intercalate into the base pairs of DNA, and there is an electrostatic binding along with intercalation binding.
学科主题	Instruments & Instrumentation ; Spectroscopy
语种	英语
WOS记录号	WOS:000282855500008
资助机构	National Natural Science Foundation of China [30572254] ; National Natural Science Foundation of China [30572254] ; National Natural Science Foundation of China [30572254] ; National Natural Science Foundation of China [30572254]
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/18409]
专题	成都生物研究所_天然产物研究
通讯作者	Wang, XM, SW Univ Sci & Technol, Sch Mat Sci & Engn, Mianyang 621010, Peoples R China
推荐引用方式 GB/T 7714	Huang, Jianhang,Wang, Xingming,Fei, Dan,et al. Interactions of Vitamin K-3 with Herring-Sperm DNA Using Spectroscopy and Electrochemistry[J]. APPLIED SPECTROSCOPY,2010,64(10):1126-1130.
APA	Huang, Jianhang,Wang, Xingming,Fei, Dan,Ding, Lisheng,&Wang, XM, SW Univ Sci & Technol, Sch Mat Sci & Engn, Mianyang 621010, Peoples R China.(2010).Interactions of Vitamin K-3 with Herring-Sperm DNA Using Spectroscopy and Electrochemistry.APPLIED SPECTROSCOPY,64(10),1126-1130.
MLA	Huang, Jianhang,et al."Interactions of Vitamin K-3 with Herring-Sperm DNA Using Spectroscopy and Electrochemistry".APPLIED SPECTROSCOPY 64.10(2010):1126-1130.