Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study

	Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study
	Chen, J; Lan, P; Tarr, A; Yan, YM; Francki, M; Appels, R; Ma, W; Ma, W, Murdoch Univ, Ctr Food & Genome Med, Western Australia Dept Agr & Food, State Agr Biotechnol Ctr,Mol Plant Breeding CRC, South St, Perth, WA 6150, Australia.
刊名	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
	2007
卷号	21 期号:17 页码:2913_2917
ISSN号	0951-4198
产权排序	2
英文摘要	Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instrumentation has been used to analyze wheat seed gliadins as an alternative to other established methods, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), capillary electrophoresis (CE), high-performance liquid chromatography (HPLC), etc. The MALDI-TOF approach has shown to have many advantages such as high resolution, cost effectiveness and high throughput. MALDITOF-based gliadin profiles have been used for fast wheat cultivar identification. However, the genetic information represented by individual gliadin peaks has not been utilized. In this study a wheat doubled haploid population with a genetic linkage map of good coverage was used to assay individual gliadin peaks from MALDI-TOF profiles as molecular markers. Eight segregating peaks in the population were scored as polymorphic across the population. The 1 to 1 segregating ratios validated the scoring of the peaks and all peaks were mapped to the expected chromosomes or linkage groups on the available linkage map: 1 peak on chromosome 1A, 1 on 6A, 4 on 6B and 2 on 61). Copyright (c) 2007 John Wiley & Sons, Ltd.
学科主题	Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
语种	英语
WOS记录号	WOS:000249074200014
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/17949]
专题	成都生物研究所_天然产物研究
通讯作者	Ma, W, Murdoch Univ, Ctr Food & Genome Med, Western Australia Dept Agr & Food, State Agr Biotechnol Ctr,Mol Plant Breeding CRC, South St, Perth, WA 6150, Australia.
推荐引用方式 GB/T 7714	Chen, J,Lan, P,Tarr, A,et al. Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2007,21(17):2913_2917.
APA	Chen, J.,Lan, P.,Tarr, A.,Yan, YM.,Francki, M.,...&Ma, W, Murdoch Univ, Ctr Food & Genome Med, Western Australia Dept Agr & Food, State Agr Biotechnol Ctr,Mol Plant Breeding CRC, South St, Perth, WA 6150, Australia..(2007).Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,21(17),2913_2917.
MLA	Chen, J,et al."Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 21.17(2007):2913_2917.