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	Abnormal Gene Expression in Ovaries and Gonadal adipose tissue in a DHT-induced Polycystic Ovary Syndrome (PCOS) Mouse model
	Li-Feng SUN; Ya-Li YANG; Yan-YU; Tian-Xia XIAO; Meng-Xia LI; Hui-Ru TANG; Jian V. ZHANG
	2017
会议日期	2017
会议地点	合肥
英文摘要	Polycystic ovary (or ovarian) syndrome (PCOS) is one of the most common endocrine abnormalities in women of reproductive age. It affects approximately 10% of women worldwide. Since it has high morbidity and multiple phenotypes, PCOS seriously affects the health and life of patients. Although there is a substantial amount of published data referring to the pathogenesis and pathology of PCOS, e.g., studies describing the important role of insulin in this syndrome, current knowledge on the prevention, diagnosis and cure of PCOS is unsatisfactory. It is necessary to ascertain etiological factors, clarify pathological mechanisms, screen diagnostic markers and discover useful therapeutic targets for PCOS. Methods: In this study, a dihydrotestosterone (DHT)-induced PCOS mouse model was generated by using subcutaneous implantable release capsules. Following this, body parameters, including body weight, estrous cycle, glucose tolerance, serum hormone level, ovarian tissue morphology and gonadal adipose tissue morphology, were measured.After DHT treatment (empty capsules as control), ovaries and gonadal fat from the mice were used for gene expression analysis via AffymetrixGeneChip® Mouse Transcriptome Assay 1.0 array. Results: The symptoms of PCOS, such as hyperandrogenemia, tendency toward obesity, blood glucose regulation disorder, and polycystic ovaries, could be observed in the DHT-treated mice.Then, microarray data analysis was used to filter genes affected in the DHT-treated mouse tissues based on functional annotation and clustering analysis (Q<0.05, P< 0.01, Fold change >2). It was found that a total of 75 messenger ribonucleic acids (mRNAs) and 13 long-non-coding ribonucleic acids (lncRNAs) were differentially expressed between the DHT-treated ovaries and control ovaries. The function of these coding genes was related to cell proliferation, apoptosis and oxidation-reduction processes, among others. Comparing the DHT-treated gonadal fat with control fat, 122 mRNA and 50 lncRNA were found to be differentially expressed. The functions of these genes have been mainly reported to be involved in lipid metabolic processes, cell proliferation, etc.Conclusion: This study is important for the elucidation of PCOS pathology and discovery of therapeutic targets. Chronic DHT treatment could induce PCOS-like symptoms in mice, including blood glucose disorder and polycystic ovaries. Meanwhile, cell hypertrophy was commonly observed in the DHT-treated mouse ovaries and gonadal adipose tissues. Although many genes involving lipid metabolism displayed altered expression, none of the genes were common in both types of tissues. It is necessary to explore common effectors in the two kinds of tissues that are affected by DHT. However, in this study, many lncRNA were also found to be altered, and their functions are poorly understood. These may be the key factors in answering the questions on PCOS.
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12417]
专题	深圳先进技术研究院_医药所
作者单位	2017
推荐引用方式 GB/T 7714	Li-Feng SUN,Ya-Li YANG,Yan-YU,et al. Abnormal Gene Expression in Ovaries and Gonadal adipose tissue in a DHT-induced Polycystic Ovary Syndrome (PCOS) Mouse model[C]. 见:. 合肥. 2017.

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