'Obligate' anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice.

	'Obligate' anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice.
	Yang, Mei; Ling, Chang-Chun; Lin, Qiu-Bin; Geng, Wei; Shi, Lei; Yu, Bin; Li, Chang-Xian; Man, Kwan; Huang, Jian-Dong; Ng, Kevin T. P
刊名	ONCOLOGY LETTERS
	2017
文献子类	期刊论文
英文摘要	The antitumor properties of bacteria have been demonstrated over the past decades. However, the efficacy is limited and unclear. Furthermore, systemic infection remains a serious concern in bacteria treatment. In this study, the effect of YB1, a rationally designed 'obligate' anaerobic Salmonella typhimurium strain, on liver tumor growth and metastasis in a nude mouse orthotopic liver tumor model was investigated. The orthotopic liver tumor model was established in nude mice using the hepatocellular carcinoma cell line MHCC-97L. Two weeks after orthotopic liver tumor implantation, YB1, SL7207 and saline were respectively administered through the tail vein of the mice. Longitudinal monitoring of tumor growth and metastasis was performed using Xenogen IV IS, and direct measurements of tumor volume were taken 3 weeks after treatment. In vitro, MHCC-97L and PLC cells were incubated with YB1 or SL7207 under anaerobic conditions. YB1 was observed to invade tumor cells and induce tumor cell apoptosis and death. The results revealed that all mice in the YB1 group were alive 3 weeks after YB1 injection while all mice in the SL7207 group died within 11 days of the SL7207 injection. The body weight decreased by similar to 9% on day 1 after YB1 injection and but subsequently recovered. Liver tumor growth and metastases were significantly inhibited following YB1 treatment. By contrast to the control group, a large number of Grl-positive cells were detected on days 1 to 21 following YB1 treatment. Furthermore, YB1 also effectively invaded tumor cells and induced tumor cell apoptosis and death. In conclusion, YB1 suppressed liver tumor growth and metastasis in a nude mice liver tumor model. The potential mechanism may be through enhancing innate immune response and inducing tumor cell apoptosis and cell death.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12323]
专题	深圳先进技术研究院_医药所
作者单位	ONCOLOGY LETTERS
推荐引用方式 GB/T 7714	Yang, Mei,Ling, Chang-Chun,Lin, Qiu-Bin,et al. 'Obligate' anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice.[J]. ONCOLOGY LETTERS,2017.
APA	Yang, Mei.,Ling, Chang-Chun.,Lin, Qiu-Bin.,Geng, Wei.,Shi, Lei.,...&Ng, Kevin T. P.(2017).'Obligate' anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice..ONCOLOGY LETTERS.
MLA	Yang, Mei,et al."'Obligate' anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice.".ONCOLOGY LETTERS (2017).