Presence of Bacteria in Spontaneous Achilles Tendon Ruptures.

	Presence of Bacteria in Spontaneous Achilles Tendon Ruptures.
	Rolf, Christer G.; Fu, Sai-Chuen; Hopkins, Chelsea; Luan, Ju; Ip, Margaret; Yung, Shu-Hang; Friman, Goran; Qin, Ling; Chan, Kai-Ming
刊名	AMERICAN JOURNAL OF SPORTS MEDICINE
	2017
文献子类	期刊论文
英文摘要	Background: The structural pathology of Achilles tendon (AT) ruptures resembles tendinopathy, but the causes remain unknown. Recently, a number of diseases were found to be attributed to bacterial infections, resulting in low-grade inflammation and progressive matrix disturbance. The authors speculate that spontaneous AT ruptures may also be influenced by the presence of bacteria. Hypothesis: Bacteria are present in ruptured ATs but not in healthy tendons. Study Design: Cross-sectional study; Level of evidence, 3. Methods: Patients with spontaneous AT ruptures and patients undergoing anterior cruciate ligament (ACL) reconstruction were recruited for this study. During AT surgical repair, excised tendinopathic tissue was collected, and healthy tendon samples were obtained as controls from hamstring tendon grafts used in ACL reconstruction. Half of every sample was reserved for DNA extraction and the other half for histology. Polymerase chain reaction (PCR) was conducted using 16S rRNA gene universal primers, and the PCR products were sequenced for the identification of bacterial species. A histological examination was performed to compare tendinopathic changes in the case and control samples. Results: Five of 20 AT rupture samples were positive for the presence of bacterial DNA, while none of the 23 hamstring tendon samples were positive. Sterile operating and experimental conditions and tests on samples, controlling for harvesting and processing procedures, ruled out the chance of postoperative bacterial contamination. The species identified predominantly belonged to the Staphylococcus genus. AT rupture samples exhibited histopathological features characteristic of tendinopathy, and most healthy hamstring tendon samples displayed normal tendon features. There were no apparent differences in histopathology between the bacterial DNA-positive and bacterial DNA-negative AT rupture samples. Conclusion: The authors have demonstrated the presence of bacterial DNA in ruptured AT samples. It may suggest the potential involvement of bacteria in spontaneous AT ruptures.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12144]
专题	深圳先进技术研究院_医工所
作者单位	AMERICAN JOURNAL OF SPORTS MEDICINE
推荐引用方式 GB/T 7714	Rolf, Christer G.,Fu, Sai-Chuen,Hopkins, Chelsea,et al. Presence of Bacteria in Spontaneous Achilles Tendon Ruptures.[J]. AMERICAN JOURNAL OF SPORTS MEDICINE,2017.
APA	Rolf, Christer G..,Fu, Sai-Chuen.,Hopkins, Chelsea.,Luan, Ju.,Ip, Margaret.,...&Chan, Kai-Ming.(2017).Presence of Bacteria in Spontaneous Achilles Tendon Ruptures..AMERICAN JOURNAL OF SPORTS MEDICINE.
MLA	Rolf, Christer G.,et al."Presence of Bacteria in Spontaneous Achilles Tendon Ruptures.".AMERICAN JOURNAL OF SPORTS MEDICINE (2017).