Surface-enrichment with hydroxyapatite nanoparticles in stereolithography-fabricated composite polymer scaffolds promotes bone repair.

	Surface-enrichment with hydroxyapatite nanoparticles in stereolithography-fabricated composite polymer scaffolds promotes bone repair.
	Guillaume, O.; Geven, M. A.; Sprecher, C. M.; Stadelmann, V. A.; Grijpma, D. W.; Tang, T. T.; Qin, L.; Lai, Y.; Alini, M.; de Bruijn, J. D.
刊名	ACTA BIOMATERIALIA
	2017
文献子类	期刊论文
英文摘要	Fabrication of composite scaffolds using stereolithography (SLA) for bone tissue engineering has shown great promises. However, in order to trigger effective bone formation and implant integration, exogenous growth factors are commonly combined to scaffold materials. In this study, we fabricated biodegradable composite scaffolds using SLA and endowed them with osteopromotive properties in the absence of biologics. First we prepared photo-crosslinkable poly(trimethylene carbonate) (PTMC) resins containing 20 and 40 wt% of hydroxyapatite (HA) nanoparticles and fabricated scaffolds with controlled macro architecture. Then, we conducted experiments to investigate how the incorporation of HA in photo-crosslinked PTMC matrices improved human bone marrow stem cells osteogenic differentiation in vitro and kinetic of bone healing in vivo. We observed that bone regeneration was significantly improved using composite scaffolds containing as low as 20 wt% of HA, along with difference in terms of osteogenesis and degree of implant osseointegration. Further investigations revealed that SLA process was responsible for the formation of a rich microscale layer of HA corralling scaffolds. To summarize, this work is of substantial importance as it shows how the fabrication of hierarchical biomaterials via surface enrichment of functional HA nanoparticles in composite polymer stereolithographic structures could impact in vitro and in vivo osteogenesis. Statement of Significance This study reports for the first time the enhance osteopromotion of composite biomaterials, with controlled macro-architecture and microscale distribution of hydroxyapatite particles, manufactured by stereolithography. In this process, the hydroxyapatite particles are not only embedded into an erodible polymer matrix, as reported so far in the literature, but concentrated at the surface of the structures. This leads to robust in vivo bone formation at low concentration of hydroxyapatite. The reported 3D self corralling composite architecture provides significant opportunities to develop functional biomaterials for bone repair and tissue engineering. (C) 2017 Published by Elsevier Ltd on behalf of Acta Materialia Inc.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12142]
专题	深圳先进技术研究院_医工所
作者单位	ACTA BIOMATERIALIA
推荐引用方式 GB/T 7714	Guillaume, O.,Geven, M. A.,Sprecher, C. M.,et al. Surface-enrichment with hydroxyapatite nanoparticles in stereolithography-fabricated composite polymer scaffolds promotes bone repair.[J]. ACTA BIOMATERIALIA,2017.
APA	Guillaume, O..,Geven, M. A..,Sprecher, C. M..,Stadelmann, V. A..,Grijpma, D. W..,...&Eglin, D..(2017).Surface-enrichment with hydroxyapatite nanoparticles in stereolithography-fabricated composite polymer scaffolds promotes bone repair..ACTA BIOMATERIALIA.
MLA	Guillaume, O.,et al."Surface-enrichment with hydroxyapatite nanoparticles in stereolithography-fabricated composite polymer scaffolds promotes bone repair.".ACTA BIOMATERIALIA (2017).