Visualization and Quantification of Browning Using a Ucp1-2A-Luciferase Knock-in Mouse Model.

	Visualization and Quantification of Browning Using a Ucp1-2A-Luciferase Knock-in Mouse Model.
	Mao, Liufeng; Nie, Baoming; Nie, Tao; Hui, Xiaoyan; Gao, Xuefei; Lin, Xiaoliang; Liu, Xin; Xu, Yong; Tang, Xiaofeng; Yuan, Ran
刊名	DIABETES
	2017
文献子类	期刊论文
英文摘要	Both mammals and adult humans possess classic brown adipocytes and beige adipocytes, and the amount and activity of these adipocytes are considered key factors in combating obesity and its associated metabolic diseases. Uncoupling protein 1 (Ucp1) is the functional marker of both brown and beige adipocytes. To facilitate a reliable, easy, and sensitive measurement of Ucp1 expression both in vivo and in vitro, we generated a Ucp1-2A-luciferase knock-inmouse by deleting the stop codon for the mouse Ucp1 gene and replacing it with a 2A peptide. This peptide was followed by the luciferase coding sequence to recapitulate the expression of the Ucp1 gene at the transcriptional and translational levels. With this mouse, we discovered a cold-sensitive brown/beige adipose depot underneath the skin of the ears, which we named uBAT. Because of the sensitivity and high dynamic range of luciferase activity, the Ucp1-2A-luciferase mouse is useful for both in vitro quantitative determination and in vivo visualization of nonshivering thermogenesis. With the use of this model, we identified and characterized axitinib, an oral small-molecule tyrosine kinase inhibitor, as an effective browning agent.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12044]
专题	深圳先进技术研究院_医工所
作者单位	DIABETES
推荐引用方式 GB/T 7714	Mao, Liufeng,Nie, Baoming,Nie, Tao,et al. Visualization and Quantification of Browning Using a Ucp1-2A-Luciferase Knock-in Mouse Model.[J]. DIABETES,2017.
APA	Mao, Liufeng.,Nie, Baoming.,Nie, Tao.,Hui, Xiaoyan.,Gao, Xuefei.,...&Wu, Donghai.(2017).Visualization and Quantification of Browning Using a Ucp1-2A-Luciferase Knock-in Mouse Model..DIABETES.
MLA	Mao, Liufeng,et al."Visualization and Quantification of Browning Using a Ucp1-2A-Luciferase Knock-in Mouse Model.".DIABETES (2017).