Single-Molecule Analysis of MicroRNA and Logic Operations Using a Smart Plasmonic Nanobiosensor

doi:10.1021/jacs.7b12772

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Single-Molecule Analysis of MicroRNA and Logic Operations Using a Smart Plasmonic Nanobiosensor
	Zhang, Y ; Shuai, ZH ; Zhou, H ; Luo, ZM ; Liu, B ; Zhang, YN ; Zhang, L ; Chen, SF ; Chao, J ; Weng, LM
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2018
卷号	140 期号:11 页码:3988-3993
关键词	Core-shell Nanocubes Silver Nanoparticles Gold Nanoparticles Dna Nanostructure Au Surface Nanoprobes Biosensors Platform Sensors
ISSN号	0002-7863
DOI	10.1021/jacs.7b12772
文献子类	期刊论文
英文摘要	Analysis of biomolecules at the single-molecule level is a great challenge in molecular diagnostics, gene profiling, and environmental monitoring. In this work, we design a smart plasmonic nanobiosensor based on individual Au@Ag core-shell nanocube (Au@Ag NC) modified with tetrahedron-structured DNA (tsDNA) for detecting microRNA 21 (miR-21) at the single-molecule level. An average localized surface plasmon resonance (LSPR) scattering spectral wavelength shift of approximately 0.4 nm can be obtained for a single miR-21 hybridization event on the nanobiosensor. In addition, the sensing mechanism of the individual Au@Ag NC is further verified by the three-dimensional finite-difference time-domain (3D-FDTD) simulations. Notably, this system not only allows the real-time detection of miR-21 with an aM level sensitivity over a large dynamic range from 1 aM to 1 nM, but also enables DNA-based logic operations as well as biomemory by exploiting miR-21, KpnI, and StuI-responsive assays. Our study opens a unique method for single-molecule detection of biomolecules and thus holds great promise in a variety of biological and biomedical applications.
语种	英语
WOS记录号	WOS:000428356000028
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/29138]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Zhang, Y 2.Shuai, ZH 3.Zhou, H 4.Luo, ZM 5.Liu, B 6.Zhang, YN 7.Zhang, L 8.Chen, SF 9.Chao, J 10.Weng, LM
推荐引用方式 GB/T 7714	Zhang, Y,Shuai, ZH,Zhou, H,et al. Single-Molecule Analysis of MicroRNA and Logic Operations Using a Smart Plasmonic Nanobiosensor[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2018,140(11):3988-3993.
APA	Zhang, Y.,Shuai, ZH.,Zhou, H.,Luo, ZM.,Liu, B.,...&Wang, LH.(2018).Single-Molecule Analysis of MicroRNA and Logic Operations Using a Smart Plasmonic Nanobiosensor.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,140(11),3988-3993.
MLA	Zhang, Y,et al."Single-Molecule Analysis of MicroRNA and Logic Operations Using a Smart Plasmonic Nanobiosensor".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 140.11(2018):3988-3993.