NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA

doi:10.1016/j.dnarep.2018.03.005

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA
	Deng, YJ ; Feng, L ; Zhou, H ; Xiao, X ; Wang, FP ; Liu, XP
刊名	DNA REPAIR
	2018
卷号	65 页码:54-63
关键词	Prime Transcription Initiation Exoribonuclease Rnase-r Escherichia-coli Crystal-structure Messenger-rna Mycobacterium-tuberculosis Bacillus-subtilis Hyperthermophilic Archaea Oligoribonuclease Degradation
ISSN号	1568-7864
DOI	10.1016/j.dnarep.2018.03.005
文献子类	期刊论文
英文摘要	In cells, degrading DNA and RNA by various nucleases is very important. These processes are strictly controlled and regulated to maintain DNA integrity and to mature or recycle various RNAs. NanoRNase (Nrn) is a 3'exonuclease that specifically degrades nanoRNAs shorter than 5 nucleotides. Several Nrns have been identified and characterized in bacteria, mainly in Firmicutes. Archaea often grow in extreme environments and might be subjected to more damage to DNA/RNA, so DNA repair and recycling of damaged RNA are very important in archaea. There is no report on the identification and characterization of Nrn in archaea. Aeropyrum pernix encodes three potential Nrns: NrnA (Ape1437), NrnB (Ape0124), and an Nrn-like protein Ape2190. Biochemical characterization showed that only Ape0124 could degrade ssDNA and ssRNA from the 3'-end in the presence of Mn2+. Interestingly, unlike bacterial Nrns, Ape0124 prefers ssDNA, including short nanoDNA, and degrades nanoRNA with lower efficiency. The 3'-DNA backbone was found to be required for efficiently hydrolyzing the phosphodiester bonds. In addition, Ape0124 also degrads the 3'-overhang of double-stranded DNA. Interestingly, Ape0124 could hydrolyze pAp into AMP, which is a feature of bacterial NrnA, not NrnB. Our results indicate that Ape0124 is a novel Nrn with a combined substrate profile of bacterial NrnA and NrnB.
语种	英语
WOS记录号	WOS:000432617300008
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/29080]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Deng, YJ 2.Feng, L 3.Zhou, H 4.Xiao, X 5.Wang, FP 6.Liu, XP
推荐引用方式 GB/T 7714	Deng, YJ,Feng, L,Zhou, H,et al. NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA[J]. DNA REPAIR,2018,65:54-63.
APA	Deng, YJ,Feng, L,Zhou, H,Xiao, X,Wang, FP,&Liu, XP.(2018).NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA.DNA REPAIR,65,54-63.
MLA	Deng, YJ,et al."NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA".DNA REPAIR 65(2018):54-63.