Inert Gas Deactivates Protein Activity by Aggregation

doi:10.1038/s41598-017-10678-3

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Inert Gas Deactivates Protein Activity by Aggregation
	Zhang, LJ; Zhang, YB; Cheng, J; Wang, L; Wang, XY; Zhang, M; Gao, Y; Hu, J; Zhang, XH; Lu, JH
刊名	SCIENTIFIC REPORTS
	2017
卷号	7 期号:-页码:-
关键词	Der-waals Complexes Xenon Binding Xe Myoglobin Pathways Nitrogen Cavity Kr Ionization
ISSN号	2045-2322
DOI	10.1038/s41598-017-10678-3
文献子类	期刊论文
英文摘要	Biologically inert gases play important roles in the biological functionality of proteins. However, researchers lack a full understanding of the effects of these gases since they are very chemically stable only weakly absorbed by biological tissues. By combining X-ray fluorescence, particle sizing and molecular dynamics (MD) simulations, this work shows that the aggregation of these inert gases near the hydrophobic active cavity of pepsin should lead to protein deactivation. Micro X-ray fluorescence spectra show that a pepsin solution can contain a high concentration of Xe or Kr after gassing, and that the gas concentrations decrease quickly with degassing time. Biological activity experiments indicate a reversible deactivation of the protein during this gassing and degassing. Meanwhile, the nanoparticle size measurements reveal a higher number of "nanoparticles" in gas-containing pepsin solution, also supporting the possible interaction between inert gases and the protein. Further, MD simulations indicate that gas molecules can aggregate into a tiny bubble shape near the hydrophobic active cavity of pepsin, suggesting a mechanism for reducing their biological function.
WOS关键词	DER-WAALS COMPLEXES ; XENON ; BINDING ; XE ; MYOGLOBIN ; PATHWAYS ; NITROGEN ; CAVITY ; KR ; IONIZATION
语种	英语
WOS记录号	WOS:000408781200035
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/28697]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zhang, LJ,Zhang, YB,Cheng, J,et al. Inert Gas Deactivates Protein Activity by Aggregation[J]. SCIENTIFIC REPORTS,2017,7(-):-.
APA	Zhang, LJ.,Zhang, YB.,Cheng, J.,Wang, L.,Wang, XY.,...&Fang, HP.(2017).Inert Gas Deactivates Protein Activity by Aggregation.SCIENTIFIC REPORTS,7(-),-.
MLA	Zhang, LJ,et al."Inert Gas Deactivates Protein Activity by Aggregation".SCIENTIFIC REPORTS 7.-(2017):-.