Dual-mode electrochemical analysis of microRNA-21 using gold nanoparticle-decorated MoS2 nanosheet

doi:10.1016/j.bios.2017.03.040

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Dual-mode electrochemical analysis of microRNA-21 using gold nanoparticle-decorated MoS2 nanosheet
	Su, S; Cao, WF; Liu, W; Lu, ZW; Zhu, D; Chao, J; Weng, LX; Wang, LH; Fan, CH; Wang, LH
刊名	BIOSENSORS & BIOELECTRONICS
	2017
卷号	94 期号:-页码:552-559
关键词	Graphene Oxide Signal Amplification Mos2/graphene Composites Breast-cancer Dna Nanostructure Biosensor Sensor Cells Strategy
ISSN号	0956-5663
DOI	10.1016/j.bios.2017.03.040
文献子类	期刊论文
英文摘要	The detection of microRNA plays an important role in early cancer diagnosis. Herein, a dual-mode electronic biosensor was developed for microRNA-21 (miRNA-21) detection based on gold nanoparticle-decorated MoS2 nanosheet (AuNPs@MoS2). A classical DNA "sandwich" structure was employed to construct MoS2-based electrochemical sensor, including capture DNA, target miRNA-21 and DNA-modified nanoprobe. [Fe(CN)(6)](3-/4-) and [Ru(NH3)(6)](3+) were selected as electrochemical indicators to monitor the preparation process and evaluate the performance of MoS2-based electrochemical biosensor by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV), respectively. Such MoS2-based biosensor exhibited excellent performance for miRNA-21 detection in the range from 10 fM to 1 nM with detection limit of 0.78 fM and 0.45 fM for DPV and EIS technique, respectively. Furthermore, the proposed MoS2-based biosensor displayed high selectivity and stability, which could be used to determine miRNA-21 in human serum samples with satisfactory results. All data suggested that such MoS2-based nanocomposite may be a potential candidate for biosensing ranging from nucleic acid to protein detection.
WOS关键词	GRAPHENE OXIDE ; SIGNAL AMPLIFICATION ; MOS2/GRAPHENE COMPOSITES ; BREAST-CANCER ; DNA ; NANOSTRUCTURE ; BIOSENSOR ; SENSOR ; CELLS ; STRATEGY
语种	英语
WOS记录号	WOS:000401201300073
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/28619]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Su, S,Cao, WF,Liu, W,et al. Dual-mode electrochemical analysis of microRNA-21 using gold nanoparticle-decorated MoS2 nanosheet[J]. BIOSENSORS & BIOELECTRONICS,2017,94(-):552-559.
APA	Su, S.,Cao, WF.,Liu, W.,Lu, ZW.,Zhu, D.,...&Wang, LH.(2017).Dual-mode electrochemical analysis of microRNA-21 using gold nanoparticle-decorated MoS2 nanosheet.BIOSENSORS & BIOELECTRONICS,94(-),552-559.
MLA	Su, S,et al."Dual-mode electrochemical analysis of microRNA-21 using gold nanoparticle-decorated MoS2 nanosheet".BIOSENSORS & BIOELECTRONICS 94.-(2017):552-559.