Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity

doi:10.1021/acsami.7b03987

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity
	Zhao, RT; Lv, M; Li, Y; Sun, MX; Kong, W; Wang, LH; Song, SP; Fan, CH; Jia, LL; Qiu, SF
刊名	ACS APPLIED MATERIALS & INTERFACES
	2017
卷号	9 期号:18 页码:15328-15341
关键词	Graphene Oxide Pegylation Silver Nanoparticles Antibacterial Activity Antibiotic Resistance Stability Long-term Effectiveness
ISSN号	1944-8244
DOI	10.1021/acsami.7b03987
文献子类	期刊论文
英文摘要	The increasing occurrence of antibiotic-resistant pathogens, especially superbugs, is compromising the efficacy of traditional antibiotics. Silver nanoparticles (AgNPs) loaded graphene oxide (GO) nanocomposite (GO-Ag) has drawn great interest as a promising alternative antibacterial material. However, GO-Ag nanocomposite often irreversibly aggregates in physiological solutions, severely influencing its antibacterial capacity and practical application. Herein, a PEGylated and AgNPs loaded GO nanocomposite (GOPEG-Ag) is synthesized through a facile approach utilizing microwave irradiation, while avoiding extra reducing agents. Through PEGylation, the synthesized GO-PEG-Ag nano composite dispersed stably over one month in a series of media and resisted centrifugation at 10 000xg for 5 min, which would benefit effective contact between the nanocomposite and the bacteria. In contrast, GO-Ag aggregated within 1 h of dispersion in physiological solutions. In comparison with GO-Ag, GO-PEG-Ag showed stronger bactericidal capability toward not only normal Gram-negative/positive bacteria such as E. coli and S. aureus (similar to 100% of E. coli and similar to 95.3% of S. aureus reduction by 10 mu g/mL nanocomposite for 2.5 h), but also superbugs. Moreover, GO-PEG-Ag showed lower cytotoxicity toward HeLa cells. Importantly, GO-PEG-Ag presented long-term antibacterial effectiveness, remaining similar to 95% antibacterial activity after one-week storage in saline solution versus <35% for GO-Ag. The antibacterial mechanisms of GO-PEG-Ag were evidenced as damage to the bacterial structure and production of reactive oxygen species, causing cytoplasm leakage and metabolism decrease. The stable GO-PEG-Ag nanocomposite with powerful and long-term antibacterial capability provides a more practical and effective strategy for fighting superbugs-including pathogen threats in biomedicine and public health.
语种	英语
WOS记录号	WOS:000401307100013
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/27448]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zhao, RT,Lv, M,Li, Y,et al. Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(18):15328-15341.
APA	Zhao, RT.,Lv, M.,Li, Y.,Sun, MX.,Kong, W.,...&Hao, RZ.(2017).Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity.ACS APPLIED MATERIALS & INTERFACES,9(18),15328-15341.
MLA	Zhao, RT,et al."Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity".ACS APPLIED MATERIALS & INTERFACES 9.18(2017):15328-15341.