Expression and radiolabeling of Cas9 protein

doi:10.1007/s41365-016-0165-8

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Expression and radiolabeling of Cas9 protein
	Yan, QL; Kong, HT; Xia, K; Zhang, Y; Aladlbahi, A; Shi, JY; Wang, LH; Fan, CH; Zhao, Y; Zhu, Y
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2017
卷号	28 期号:1 页码:-
关键词	Cas9 Radiolabeling I-125 Labeling Yield Stability
ISSN号	1001-8042
DOI	10.1007/s41365-016-0165-8
文献子类	期刊论文
英文摘要	As a robust platform for genome editing, CRISPR/Cas9 is currently being explored for engineering biology or therapeutics, yet means for quantitative detection of Cas9 proteins remain to be fully realized. Here, we expressed Cas9 proteins and developed a novel detection method that traced Cas9 based on radiolabeled iodine. Through optimizing the reaction conditions of reaction time, temperature and cycles, we obtained I-125-Cas9 of high labeling yield. The prepared I-125-Cas9 was stable in various media and preserved excellent genome editing efficiency. Thus, our strategy provides a convenient and efficient tool for further tracing biological behaviors of Cas9 proteins in living systems.
语种	英语
WOS记录号	WOS:000393150100007
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/27319]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Yan, QL,Kong, HT,Xia, K,et al. Expression and radiolabeling of Cas9 protein[J]. NUCLEAR SCIENCE AND TECHNIQUES,2017,28(1):-.
APA	Yan, QL.,Kong, HT.,Xia, K.,Zhang, Y.,Aladlbahi, A.,...&Zhu, Y.(2017).Expression and radiolabeling of Cas9 protein.NUCLEAR SCIENCE AND TECHNIQUES,28(1),-.
MLA	Yan, QL,et al."Expression and radiolabeling of Cas9 protein".NUCLEAR SCIENCE AND TECHNIQUES 28.1(2017):-.