Triplex-quadruplex structural scaffold: a new binding structure of aptamer

	Triplex-quadruplex structural scaffold: a new binding structure of aptamer
	Bing, Tao 1,2; Zheng, Wei 1; Zhang, Xin 1,2; Shen, Luyao 1,2; Liu, Xiangjun 1,2; Wang, Fuyi 1,2; Cui, Jie 1; Cao, Zehui 1; Shangguan, Dihua 1,2
刊名	SCIENTIFIC REPORTS
	2017-11-13
卷号	7
英文摘要	Apart from the canonical Watson-Crick duplex, nucleic acids can often form other structures, e.g. G-quadruplex and triplex. These structures give nucleic acid additional functions besides coding for genetic information. Aptamers are one type of functional nucleic acids that bind to specific targets with high selectivity and affinity by folding into special tertiary structures. Despite the fact that numerous aptamers have been reported, only a few different types of aptamer structures are identified. Here we report a novel triplex-quadruplex hybrid scaffold formed by a codeine binding aptamer (CBA). CBA and its derivatives are G-rich DNA sequences. Codeine binding can induce the formation of a complex structure for this aptamer containing a G-quadruplex and a G.GC triplex, while codeine is located at the junction of the triplex and quadruplex. When split CBA into two moieties, codeine does not bind either moieties individually, but can bind them together by inducing the formation of the triplex-quadruplex scaffold. This structure formation induced by codeine binding is shown to inhibit polymerase reaction, which shows a potential application of the aptamer sequence in gene regulations.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/39169]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, CAS Res Educ Ctr Excellence Mol Sci,Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Bing, Tao,Zheng, Wei,Zhang, Xin,et al. Triplex-quadruplex structural scaffold: a new binding structure of aptamer[J]. SCIENTIFIC REPORTS,2017,7.
APA	Bing, Tao.,Zheng, Wei.,Zhang, Xin.,Shen, Luyao.,Liu, Xiangjun.,...&Shangguan, Dihua.(2017).Triplex-quadruplex structural scaffold: a new binding structure of aptamer.SCIENTIFIC REPORTS,7.
MLA	Bing, Tao,et al."Triplex-quadruplex structural scaffold: a new binding structure of aptamer".SCIENTIFIC REPORTS 7(2017).