Adaptively Recognizing Parallel-Stranded Duplex Structure for Fluorescent DNA Polarity Analysis

	Adaptively Recognizing Parallel-Stranded Duplex Structure for Fluorescent DNA Polarity Analysis
	Ye, Mei-Yun 1; Zhu, Rui-Tao 2; Lo, Xiang 3; Zhou, Xiao-Shun 1; Yin, Zheng-Zhi 4; Li, Qian 5; Shao, Yong 1
刊名	ANALYTICAL CHEMISTRY
	2017-09-05
卷号	89 期号:17 页码:8604-8608
英文摘要	Besides the canonical Watson Crick (WC) linked antiparallel-stranded duplex (aps-DNA), DNA is also able to form bioactive parallel-stranded duplex (ps-DNA) with the two involving strands adopting the equal 5'-3' polarity. Discriminating ps-DNA from aps-DNA with an ideal selectivity is more challenging because of their comparable duplex topologies. Herein, we designed a unique probe of HPIN to fluorescently recognize ps-DNA but to keep an almost nonfluorescent response in binding with aps-DNA. The success of the Hoogsteen hydrogen bonding pattern in lighting up the HPIN fluorescence over the reverse Watson Crick (rWC) one suggests the critical role of HPIN in structurally adaptive recognition to the strand polarity-determined base-pairing peculiarity. The turn on fluorescence should result from restriction of the HPIN cis/trans isomerization upon the adaptive Hoogsteen base pair binding. Such high performance in recognizing ps-DNA against aps-DNA demonstrates the promising applications of HPIN in developing unique DNA polarity-based sensors.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/39263]
专题	化学研究所_工程塑料实验室
作者单位	1.Zhejiang Normal Univ, Coll Chem & Life Sci, Inst Phys Chem, Jinhua 321004, Zhejiang, Peoples R China 2.Taiyuan Normal Univ, Dept Chem, Taiyuan 030031, Shanxi, Peoples R China 3.Langzhong Peoples Hosp, Langzhong 637400, Sichuan, Peoples R China 4.Jiaxing Univ, Coll Biol Chem Sci & Engn, Jiaxing 314001, Zhejiang, Peoples R China 5.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Ye, Mei-Yun,Zhu, Rui-Tao,Lo, Xiang,et al. Adaptively Recognizing Parallel-Stranded Duplex Structure for Fluorescent DNA Polarity Analysis[J]. ANALYTICAL CHEMISTRY,2017,89(17):8604-8608.
APA	Ye, Mei-Yun.,Zhu, Rui-Tao.,Lo, Xiang.,Zhou, Xiao-Shun.,Yin, Zheng-Zhi.,...&Shao, Yong.(2017).Adaptively Recognizing Parallel-Stranded Duplex Structure for Fluorescent DNA Polarity Analysis.ANALYTICAL CHEMISTRY,89(17),8604-8608.
MLA	Ye, Mei-Yun,et al."Adaptively Recognizing Parallel-Stranded Duplex Structure for Fluorescent DNA Polarity Analysis".ANALYTICAL CHEMISTRY 89.17(2017):8604-8608.