Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model

	Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model
	Lyu Yang 1; Zhang Ya-Wen 1; Tan Lei 1; Ji Wen-Liang 2; Yu Ping 2; Mao Lan-Qun 2; Zhou Fang 1
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2017-11-01
卷号	45 期号:11 页码:1595-1599
关键词	Ascorbic Acid Spinal Cord Injury In Vivo Microdialysis On-line Electrochemical Detection
英文摘要	Acute traumatic spinal cord injury (SCI) represents one of the most devastating injuries that afflict the human body. Ascorbic acid (AA) plays an important role in mammalian central nervous system, especially in SCI. In this study, the change of AA concentration after SCI was investigated by using an on. line electrochemical method integrated with in vivo microdialysis. A microdialysis probe (2 mm in length) was implanted into the spinal cord of an anesthetized rat (Thoracic-10). Microdialysis perfusate (2 mu L/min) was collected in the sample loop of an on-line injector for direct injection onto a glassy carbon electrode which was modified with the heat. treated single-walled carbon nanotubes (SWNTs). Normal ascorbic acid concentration in the extracellular fluids of spinal cords was (26.17 +/- 1.25) mu mol/L (n = 8). The experimental spinal cord injury, induced by a lesion at T=10, significantly increased the extracellular ascorbic acid levels to (53.24 +/- 1.95) mu mol/L (n = 8). This study provides the experimental evidence on the essential roles of ascorbic acid in spinal cord injuries.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/38466]
专题	化学研究所_活体分析化学实验室
作者单位	1.Peking Univ, Hosp 3, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Lyu Yang,Zhang Ya-Wen,Tan Lei,et al. Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(11):1595-1599.
APA	Lyu Yang.,Zhang Ya-Wen.,Tan Lei.,Ji Wen-Liang.,Yu Ping.,...&Zhou Fang.(2017).Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(11),1595-1599.
MLA	Lyu Yang,et al."Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.11(2017):1595-1599.