Using Nanoliposomes To Construct a FRET-Based Ratiometric Fluorescent Probe for Sensing Intracellular pH Values

	Using Nanoliposomes To Construct a FRET-Based Ratiometric Fluorescent Probe for Sensing Intracellular pH Values
	Zhang, Yangyang 1; Li, Shilei 1,2; Zhao, Zhenwen 1,2
刊名	ANALYTICAL CHEMISTRY
	2016-12-20
卷号	88 期号:24 页码:12380-12385
英文摘要	Nanoliposomes were first used to encapsulate two fluorophores, fluorescein and cresyl violet, to construct a FRET-based ratiometric fluorescent probe, for sensing intracellular pH values. The morphology, stability, interference factors, biocompatibility, and performance were investigated in detail. The fluorophores-encapsulated nanoliposomes (FEN) probe can be uptaken by cells within 30 min, which is faster than any other nanosensor, and cell viability was not significantly changed upon treatment with the FEN probe for 24 h, showing the low cytotoxicity and good biocompatibility of the probe. Quantitative determinations of intracellular pH of MCF-7 cells and the pH fluctuations associated with inflammation have been successfully performed with our probe. This work demonstrated that nanoliposomes could be served as an emerging approach for constructing useful fluorescent nanosensors with low toxicity.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/37959]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yangyang,Li, Shilei,Zhao, Zhenwen. Using Nanoliposomes To Construct a FRET-Based Ratiometric Fluorescent Probe for Sensing Intracellular pH Values[J]. ANALYTICAL CHEMISTRY,2016,88(24):12380-12385.
APA	Zhang, Yangyang,Li, Shilei,&Zhao, Zhenwen.(2016).Using Nanoliposomes To Construct a FRET-Based Ratiometric Fluorescent Probe for Sensing Intracellular pH Values.ANALYTICAL CHEMISTRY,88(24),12380-12385.
MLA	Zhang, Yangyang,et al."Using Nanoliposomes To Construct a FRET-Based Ratiometric Fluorescent Probe for Sensing Intracellular pH Values".ANALYTICAL CHEMISTRY 88.24(2016):12380-12385.