Interspecific Hybridization Processes between Michelia yunnanensis and M-crassipes and Embryogenesis of the Heterozygote

doi:10.21273/HORTSCI12086-17

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 资源植物与生物技术所级重点实验室

	Interspecific Hybridization Processes between Michelia yunnanensis and M-crassipes and Embryogenesis of the Heterozygote
	Xu, Haiyan 1; Li, Folian 2; Pan, Yuezhi 1; Gong, Xun1
刊名	HORTSCIENCE
	2017-08-01
卷号	52 期号:8 页码:1043-1047
关键词	Michelia Reciprocal Cross Unilateral Incompatibility Fluorescence Microscopy Cryosectioning Embryogeny
ISSN号	0018-5345
DOI	10.21273/HORTSCI12086-17
英文摘要	The investigation of hybridization processes and embryogenesis of heterozygote is an effective approach for early hybrids' identification, which could provide reliable information for successful crossbreeding. In this study, we reported the whole hybridization processes of the direct cross and reciprocal cross between Michelia yunnanensis Franch. ex Finet et Gagnep. and Michelia crassipes Law using fluorescence microscopy after aniline blue staining, with the pollen germination on stigmas, pollen tube growth in styles, and subsequent extension into the embryo sac as well as the double fertilization processes are documented in detail. The M. yunnanensis 3 M. crassipes combination displayed considerable cross-compatibility, and the heterozygote embryogenesis was further observed with an approach of modified cryosectioning technique. Besides, the whole formation processes of hybrid seeds from artificial pollination to maturation were successfully observed. However, in the reciprocal cross, we found incompatibility between pollen grains of M. yunnanensis and stigmas of M. crassipes for the reason of hysteretic identification, as well as the abnormal callose deposition which belongs to the prefertilization barriers. This is the first study in which the complete and clear hybridization processes in Michelia were reported. We inferred that unilateral incompatibility of M. crassipes detected in this study may also exist in some other Michelia species. In artificial hybridization practices, we suggest some special treatments for overcoming prefertilization barrier should be taken when treating M. crassipes as the maternal parent.
学科主题	Horticulture
语种	英语
WOS记录号	WOS:000409437100002
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/54927]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Agr Univ, Coll Hort & Landscape Architecture, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Xu, Haiyan,Li, Folian,Pan, Yuezhi,et al. Interspecific Hybridization Processes between Michelia yunnanensis and M-crassipes and Embryogenesis of the Heterozygote[J]. HORTSCIENCE,2017,52(8):1043-1047.
APA	Xu, Haiyan,Li, Folian,Pan, Yuezhi,&Gong, Xun.(2017).Interspecific Hybridization Processes between Michelia yunnanensis and M-crassipes and Embryogenesis of the Heterozygote.HORTSCIENCE,52(8),1043-1047.
MLA	Xu, Haiyan,et al."Interspecific Hybridization Processes between Michelia yunnanensis and M-crassipes and Embryogenesis of the Heterozygote".HORTSCIENCE 52.8(2017):1043-1047.