Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427
	Shin, Jin Chul 1,2; Na, Zhu 1,3,4; Lee, Dongho 5; Kim, Woncheol 1,2; Lee, Kyeong 1; Shen, Yue-Mao 3; Paik, Sang-Gi 2; Hong, Young-Soo 1; Lee, Jung Joon 1
刊名	JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
	2008-06-01
卷号	18 期号:6 页码:1101-1108
关键词	Geldanamycin Biosynthesis Streptomyces Polyketide
ISSN号	1017-7825
文献子类	Article
英文摘要	Geldanamycin and its analogs are important anticancer agents that inhibit the newly targeted heat-shock protein (Hsp) 90, which is a chaperone protein in eukaryotic cells. To resolve which geldanamycin biosynthetic genes are responsible for particular post-polyketide synthase (PKS) processing steps and in which order the reactions occur, we individually inactivated candidate genes in Streptomyces hygroscopicus subsp. duamyceticus JCM4427 and isolated and elucidated the structures of intermediates from each mutant. The results indicated that gel7 governs at least one of the benzoquinone ring oxidation steps. The gel16 was found to be involved in double-bond formation between C-4 and C-5 of 4,5-dihydrogeldanamycin, which confirmed our previous findings that this double bond is reduced during the post-PKS modification of the polyketide assembly. In addition, pro-geldanamycin, which does not possess a double bond at C-4/5, was purified from the gel7 and gel8 double-gene-inactivated mutant.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
WOS关键词	AMYCOLATOPSIS-MEDITERRANEI S699 ; BINDING SITE ; BIOSYNTHESIS ; CLUSTER ; CYTOCHROME-P450 ; IDENTIFICATION ; CLONING ; HYDROXYLASE ; RIFAMYCIN ; ACID
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
WOS记录号	WOS:000257212000016
公开日期	2011-11-24
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/1491]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.KRIBB, Taejon 305806, South Korea 2.Chungnam Natl Univ, Taejon 305764, South Korea 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 5.Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136705, South Korea
推荐引用方式 GB/T 7714	Shin, Jin Chul,Na, Zhu,Lee, Dongho,et al. Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2008,18(6):1101-1108.
APA	Shin, Jin Chul.,Na, Zhu.,Lee, Dongho.,Kim, Woncheol.,Lee, Kyeong.,...&Lee, Jung Joon.(2008).Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,18(6),1101-1108.
MLA	Shin, Jin Chul,et al."Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 18.6(2008):1101-1108.