A draft sequence of the rice (Oryza sativa ssp indica) genome

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	A draft sequence of the rice (Oryza sativa ssp indica) genome
	Yu, J; Hu, SN; Wang, J; Li, SG; Wong, KSG; Liu, B; Deng, Y; Dai, L; Zhou, Y; Zhang, XQ
刊名	CHINESE SCIENCE BULLETIN
	2001
卷号	46 期号:23 页码:1937-1942
关键词	Dna Construction Library
ISSN号	1001-6538
英文摘要	The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have produced a draft rice genome sequence of Oryza sativa ssp. indica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEMBLER, a custom-designed software package used for the initial assembly, were verified rigorously by comparisons against finished BAC clone sequences from both indica and japanica strains, available from the public databases. Over all, 96.3% of full-length cDNAs, 96.4% of STS, STR, RFLP markers, 94.0% of ESTs and 94.9% unigene clusters were identified from the draft sequence. Our preliminary analysis on the data set shows that our rice draft sequence is consistent with the comman standard accepted by the genome sequencing community. The unconditional release of the draft to the public also undoubtedly provides a fundamental resource to the international scientific communities to facilitate genomic and genetic studies on rice biology.
学科主题	Physics
URL标识	查看原文
WOS记录号	WOS:000173381900001
公开日期	2012-08-29
内容类型	期刊论文
源URL	[http://ir.itp.ac.cn/handle/311006/13631]
专题	理论物理研究所_理论物理所1978-2010年知识产出
通讯作者	Yang, HM , Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China.
推荐引用方式 GB/T 7714	Yu, J,Hu, SN,Wang, J,et al. A draft sequence of the rice (Oryza sativa ssp indica) genome[J]. CHINESE SCIENCE BULLETIN,2001,46(23):1937-1942.
APA	Yu, J.,Hu, SN.,Wang, J.,Li, SG.,Wong, KSG.,...&Yang, HM , Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China..(2001).A draft sequence of the rice (Oryza sativa ssp indica) genome.CHINESE SCIENCE BULLETIN,46(23),1937-1942.
MLA	Yu, J,et al."A draft sequence of the rice (Oryza sativa ssp indica) genome".CHINESE SCIENCE BULLETIN 46.23(2001):1937-1942.