2-(2-Phenyl-1H-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (PPIA) and its application for determination of amines by high performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/atmospheric pressure chemical ionization

	2-(2-Phenyl-1H-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (PPIA) and its application for determination of amines by high performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/atmospheric pressure chemical ionization
	You, JM ; Zhao, XN ; Suo, YR ; Wang, HL ; Li, YL ; Sun, J
刊名	analytica chimica acta
	2006-04-21
卷号	565 期号:2 页码:168-177
关键词	HPLC amines 2-(2-phenyl-1H-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (PPIA) mass spectrometry
合作状况	其它
中文摘要	a simple, sensitive, and mild method for the determination of amino compounds based on a condensation reaction with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (edc-hci) as the dehydrant with fluorescence detection has been developed. amines were derivatized to their acidamides with labeling reagent 2-(2-phenyl-1h-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (ppia). studies on derivatization conditions indicated that the coupling reaction proceeded rapidly and smoothly in the presence of a base catalyst in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) 260nm and an emission maximum at lambda(em) 380nm. the labeled derivatives exhibited high stability and were enough to be efficiently analyzed by high-performance liquid chromatography. identification of derivatives was carried out by online post-column mass spectrometry (lc/apci-ms/ms) and showed an intense protonated molecular ion corresponding m/z [mh](+) under apci in positive-ion mode. at the same time, the fluorescence properties of derivatives in various solvents or at different temperature were investigated. the method, in conjunction with a gradient elution, offered a baseline resolution of the common amine derivatives on a reversed-phase eclipse xdb-c-8 column. lc separation for the derivatized amines showed good reproducibility with acetonitrile-water as mobile phase. detection limits calculated from 0.78 pmol injection, at a signal-to-noise ratio of 3, were 3.1-18.2 fmol. the mean intra- and inter-assay precision for all amine levels were < 3.85% and 2.11%, respectively. excellent linear responses were observed with coefficients of > 0.9996. the established method for the determination of aliphatic amines from real wastewater and biological samples was satisfactory. (c) 2006 elsevier b.v. all rights reserved.
英文摘要	a simple, sensitive, and mild method for the determination of amino compounds based on a condensation reaction with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (edc-hci) as the dehydrant with fluorescence detection has been developed. amines were derivatized to their acidamides with labeling reagent 2-(2-phenyl-1h-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (ppia). studies on derivatization conditions indicated that the coupling reaction proceeded rapidly and smoothly in the presence of a base catalyst in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) 260nm and an emission maximum at lambda(em) 380nm. the labeled derivatives exhibited high stability and were enough to be efficiently analyzed by high-performance liquid chromatography. identification of derivatives was carried out by online post-column mass spectrometry (lc/apci-ms/ms) and showed an intense protonated molecular ion corresponding m/z [mh](+) under apci in positive-ion mode. at the same time, the fluorescence properties of derivatives in various solvents or at different temperature were investigated. the method, in conjunction with a gradient elution, offered a baseline resolution of the common amine derivatives on a reversed-phase eclipse xdb-c-8 column. lc separation for the derivatized amines showed good reproducibility with acetonitrile-water as mobile phase. detection limits calculated from 0.78 pmol injection, at a signal-to-noise ratio of 3, were 3.1-18.2 fmol. the mean intra- and inter-assay precision for all amine levels were < 3.85% and 2.11%, respectively. excellent linear responses were observed with coefficients of > 0.9996. the established method for the determination of aliphatic amines from real wastewater and biological samples was satisfactory. (c) 2006 elsevier b.v. all rights reserved.
学科主题	生物科学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	capillary-zone-electrophoresis ; precolumn derivatization ; 2-(9-anthryl)ethyl chloroformate ; fluorometric detection ; aliphatic-amines ; n-nitrosoamines ; waste-water ; separation ; chloride ; spectrometry
收录类别	SCI
语种	英语
WOS记录号	WOS:000237373300007
公开日期	2009-12-08
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/1334]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Shandong 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China
推荐引用方式 GB/T 7714	You, JM,Zhao, XN,Suo, YR,et al. 2-(2-Phenyl-1H-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (PPIA) and its application for determination of amines by high performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/atmospheric pressure chemical ionization[J]. analytica chimica acta,2006,565(2):168-177.
APA	You, JM,Zhao, XN,Suo, YR,Wang, HL,Li, YL,&Sun, J.(2006).2-(2-Phenyl-1H-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (PPIA) and its application for determination of amines by high performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/atmospheric pressure chemical ionization.analytica chimica acta,565(2),168-177.
MLA	You, JM,et al."2-(2-Phenyl-1H-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (PPIA) and its application for determination of amines by high performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/atmospheric pressure chemical ionization".analytica chimica acta 565.2(2006):168-177.