Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection

	Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection
	Wang, Honglun ; Suo, Yourui ; Wang, Xiaoyan ; Li, Yulin ; You, Jinmao ; Zhao, Xianen
刊名	separation and purification technology
	2007-09-15
卷号	56 期号:3 页码:371-377
关键词	supercritical carbon dioxide extraction Nitraria tangutorum Bohr. seed oil free fatty acids HPLC/APCI/MS
合作状况	其它
中文摘要	the seed oil from nitraria tangutorum samples was obtained by supercritical carbon dioxide extraction methods. the extraction parameters for this methodology, including pressure, temperature, particle size and extraction time, were optimized. the free fatty acids in the seed oil were separated with a pre-column derivation method and 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate (bdets) as a labeling regent, followed by high-performance liquid chromatography (hplc) with fluorescence detection. the target compounds were identified by mass spectrometry with atmospheric pressure chemical ionization (apci in positive-ion mode). hplc analysis shows that the main compositions of the seed oil samples were free fatty acids (ffas) in high to low concentrations as follows: linoleic acid, oleic acid, hexadecanoic acid and octadecanoic acid. the assay detection limits (at signal-to-noise of 3:1) were 3.378-6.572 nmol/l. excellent linear responses were observed, with correlation coefficients greater than 0.999. the facile bdets derivatization coupled with mass spectrometry detection allowed the development of a highly sensitive method for analyzing free fatty acids in seed oil by supercritical co2 extraction. the established method is highly efficient for seed oil extraction and extremely sensitive for fatty acid profile determination. (c) 2007 elsevier b.v. all rights reserved.
英文摘要	the seed oil from nitraria tangutorum samples was obtained by supercritical carbon dioxide extraction methods. the extraction parameters for this methodology, including pressure, temperature, particle size and extraction time, were optimized. the free fatty acids in the seed oil were separated with a pre-column derivation method and 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate (bdets) as a labeling regent, followed by high-performance liquid chromatography (hplc) with fluorescence detection. the target compounds were identified by mass spectrometry with atmospheric pressure chemical ionization (apci in positive-ion mode). hplc analysis shows that the main compositions of the seed oil samples were free fatty acids (ffas) in high to low concentrations as follows: linoleic acid, oleic acid, hexadecanoic acid and octadecanoic acid. the assay detection limits (at signal-to-noise of 3:1) were 3.378-6.572 nmol/l. excellent linear responses were observed, with correlation coefficients greater than 0.999. the facile bdets derivatization coupled with mass spectrometry detection allowed the development of a highly sensitive method for analyzing free fatty acids in seed oil by supercritical co2 extraction. the established method is highly efficient for seed oil extraction and extremely sensitive for fatty acid profile determination. (c) 2007 elsevier b.v. all rights reserved.
学科主题	生物科学
WOS标题词	science & technology ; technology
类目[WOS]	engineering, chemical
研究领域[WOS]	engineering
关键词[WOS]	co2 extraction ; simulation
收录类别	SCI
语种	英语
WOS记录号	WOS:000249250900018
公开日期	2009-12-07
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/1257]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Honglun,Suo, Yourui,Wang, Xiaoyan,et al. Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection[J]. separation and purification technology,2007,56(3):371-377.
APA	Wang, Honglun,Suo, Yourui,Wang, Xiaoyan,Li, Yulin,You, Jinmao,&Zhao, Xianen.(2007).Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection.separation and purification technology,56(3),371-377.
MLA	Wang, Honglun,et al."Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection".separation and purification technology 56.3(2007):371-377.