Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection

	Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection
	Zhao Wen-Chen 2,3; Liu Ling-Jun 2; Zhao Xian-En 1; Ding Chen-Xu 1; Suo You-Rui 1; You Jin-Mao 1,2
刊名	chinese journal of analytical chemistry
	2008-08-01
卷号	36 期号:8 页码:1071-1076
关键词	High performance liquid chromatography/mass spectrometry fluorescence detection pre-column derivatization amino acids bee pollen
合作状况	其它
中文摘要	on a reversed phase hypersil bds c-18 (200 mm x 4. 6 mm, 5 mu m) column, 20 amino acids, which were derivatized using 2-(11h-benzo [a] carbazol-11-yl) ethyl carbonochloridate (bcec-cl) as pre-column derivatization reagent, were separated in conjunction with a gradient elution. optimum derivatization was obtained by reacting of amino acids with bcec-cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. the fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. the identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. linear correlation coefficients of the amino acid derivatives were > 0.9990, and detection limits (at signal to noise of 3:1) were 1.49 - 19.74 fmol for the labeled amino acids.
英文摘要	on a reversed phase hypersil bds c-18 (200 mm x 4. 6 mm, 5 mu m) column, 20 amino acids, which were derivatized using 2-(11h-benzo [a] carbazol-11-yl) ethyl carbonochloridate (bcec-cl) as pre-column derivatization reagent, were separated in conjunction with a gradient elution. optimum derivatization was obtained by reacting of amino acids with bcec-cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. the fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. the identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. linear correlation coefficients of the amino acid derivatives were > 0.9990, and detection limits (at signal to noise of 3:1) were 1.49 - 19.74 fmol for the labeled amino acids.
学科主题	生物科学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	ionization mass-spectrometry ; 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate ; chloroformate ; identification ; hydrolysate
收录类别	SCI
语种	英语
WOS记录号	WOS:000260136800012
公开日期	2009-12-04
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/1189]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 3.Lu Nan Pharm Grp Joint Stock Ltd, Linyi 276005, Peoples R China
推荐引用方式 GB/T 7714	Zhao Wen-Chen,Liu Ling-Jun,Zhao Xian-En,et al. Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection[J]. chinese journal of analytical chemistry,2008,36(8):1071-1076.
APA	Zhao Wen-Chen,Liu Ling-Jun,Zhao Xian-En,Ding Chen-Xu,Suo You-Rui,&You Jin-Mao.(2008).Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection.chinese journal of analytical chemistry,36(8),1071-1076.
MLA	Zhao Wen-Chen,et al."Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection".chinese journal of analytical chemistry 36.8(2008):1071-1076.