Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy

doi:10.1128/AEM.00409-17

CORC > 地质与地球物理研究所 > 中国科学院地质与地球物理研究所 > 中国科学院地球与行星物理重点实验室

	Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy
	Li, Jinhua 1,2,3; Zhang, Heng 1,2,3,4; Menguy, Nicolas 3,5; Benzerara, Karim 5; Wang, Fuxian 1,2,3; Lin, Xiaoting 1,4; Chen, Zhibao 4; Pan, Yongxin 1,3
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2017-06-01
卷号	83 期号:12
关键词	Biomineralization Fluorescence-coupled Electron Microscopy Fish Magnetotactic Bacteria Tem
ISSN号	0099-2240
DOI	10.1128/AEM.00409-17
文献子类	Article
英文摘要	Magnetotactic bacteria (MTB) form intracellular chain-assembled nanocrystals of magnetite or greigite termed magnetosomes. The characterization of magnetosome crystals requires electron microscopy due to their nanoscopic sizes. However, electron microscopy does not provide phylogenetic information for MTB. We have developed a strategy for the simultaneous and rapid phylogenetic and biomineralogical characterization of uncultured MTB at the single-cell level. It consists of four steps: (i) enrichment of MTB cells from an environmental sample, (ii) 16S rRNA gene sequencing of MTB, and (iii) fluorescence in situ hybridization analyses coordinated with (iv) transmission or scanning electron microscopy of the probe-hybridized cells. The application of this strategy identified a magnetotactic Gammaproteobacteria strain, SHHR-1, from brackish sediments collected from the Shihe River estuary in Qinhuangdao City, China. SHHR-1 magnetosomes are elongated prismatic magnetites which can be idealized as hexagonal prisms. Taxonomic groups of uncultured MTB were also identified in freshwater sediments from Lake Miyun in northern Beijing via this novel coordinated fluorescence and scanning electron microscopy method based on four group-specific rRNA-targeted probes. Our analyses revealed that major magnetotactic taxonomic groups can be accurately determined only with coordinated scanning electron microscopy observations on fluorescently labeled single cells due to limited group coverage and specificity for existing group-specific MTB fluorescence in situ hybridization (FISH) probes. Our reported strategy is simple and efficient, offers great promise toward investigating the diversity and biomineralization of MTB, and may also be applied to other functional groups of microorganisms.
WOS关键词	IN-SITU HYBRIDIZATION ; CATALYZED REPORTER DEPOSITION ; MICROBIAL COMMUNITIES ; MAGNETOSOME FORMATION ; MAGNETIC-PROPERTIES ; PHYLUM NITROSPIRAE ; IDENTIFICATION ; BIOMINERALIZATION ; DIVERSITY ; PROBES
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000402386500015
资助机构	National Natural Science Foundation of China(41522402 ; National Natural Science Foundation of China(41522402 ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; 41374004 ; 41374004 ; 41330104 ; 41330104 ; 41621004) ; 41621004) ; National Natural Science Foundation of China(41522402 ; National Natural Science Foundation of China(41522402 ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; 41374004 ; 41374004 ; 41330104 ; 41330104 ; 41621004) ; 41621004) ; National Natural Science Foundation of China(41522402 ; National Natural Science Foundation of China(41522402 ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; 41374004 ; 41374004 ; 41330104 ; 41330104 ; 41621004) ; 41621004) ; National Natural Science Foundation of China(41522402 ; National Natural Science Foundation of China(41522402 ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; Open Project Support from the State Key Laboratory of Lithospheric Evolution (SKLLE, Beijing) ; 41374004 ; 41374004 ; 41330104 ; 41330104 ; 41621004) ; 41621004)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/52861]
专题	地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者	Li, Jinhua
作者单位	1.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao, Peoples R China 3.Chinese Acad Sci, France China Biomineralizat & Nanostruct Lab, Beijing, Peoples R China 4.Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing, Peoples R China 5.Sorbonne Univ, IMPMC, CNRS UMR 7590, MNHN,UPMC,IRD UMR 206, Paris, France
推荐引用方式 GB/T 7714	Li, Jinhua,Zhang, Heng,Menguy, Nicolas,et al. Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(12).
APA	Li, Jinhua.,Zhang, Heng.,Menguy, Nicolas.,Benzerara, Karim.,Wang, Fuxian.,...&Pan, Yongxin.(2017).Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(12).
MLA	Li, Jinhua,et al."Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.12(2017).