Biomineralization of a calcifying ureolytic bacterium Microbacterium sp GM-1

doi:10.1016/j.ejbt.2016.10.008

	Biomineralization of a calcifying ureolytic bacterium Microbacterium sp GM-1
	Xu, Guojing 1; Li, Dongwei 1; Jiao, Binquan 1; Li, Dou 2; Yin, Yajie3 ; Lun, Limei 1; Zhao, Ziqiang 1; Li, Shan 1
刊名	ELECTRONIC JOURNAL OF BIOTECHNOLOGY
	2017-01-15
卷号	25 页码:21-27
关键词	Calcification process Calcite precipitation Calcium carbonate precipitation Hardening of biological tissues Nickel Resistance to urea Synthesis of biominerals Urease X-ray diffraction
ISSN号	0717-3458
DOI	10.1016/j.ejbt.2016.10.008
通讯作者	Jiao, BQ (reprint author), Chongqing Univ, State Key Lab Coal Mine Disaster Dynam & Control, Chongqing 400044, Peoples R China. ; Yin, YJ (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China.
英文摘要	Background: Biomineralization is a significant process performed by living organisms in which minerals are produced through the hardening of biological tissues. Herein, we focus on calcium carbonate precipitation, as part of biomineralization, to be used in applications for environmental protection, material technology, and other fields. A strain GM-1, Microbacterium sp. GM-1, isolated from active sludge, was investigated for its ability to produce urease and induce calcium carbonate precipitation in a metabolic process. Results: It was discovered that Microbacterium sp. GM-1 resisted high concentrations of urea up to 60 g/L. In order to optimize the calcification process of Microbacterium sp. GM-1, the concentrations of Ni2+ and urea, pH value, and culture time were analyzed through orthogonal tests. The favored calcite precipitation culture conditions were as follows: the concentration of Ni2+ and urea were 50 mu M and 60 g/L, respectively, pH of 10, and culture time of 96 h. Using X-ray diffraction analysis, the calcium carbonate polymorphs produced by Microbacterium sp. GM-1 were proven to be mainly calcite. Conclusions: The results of this research provide evidence that Microbacterium sp. GM-1 can biologically induce calcification and suggest that strain GM-1 may play a potential role in the synthesis of new biominerals and in bioremediation or biorecovery. (C) 2016 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.
资助项目	Natural Science Foundation of China[51,274,262] ; Natural Science Foundation of China[31,300,025] ; Chongqing Science & Technology Commission[cstc2014jcyjA20003] ; Chongqing Science & Technology Commission[cstc2016jcyjA0390] ; Western Ph.D. project, "Westerrn light" talents cultivation plan of Chinese Academy of Sciences
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	UNIV CATOLICA DE VALPARAISO
WOS记录号	WOS:000392435900004
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/274]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Jiao, Binquan; Yin, Yajie
作者单位	1.Chongqing Univ, State Key Lab Coal Mine Disaster Dynam & Control, Chongqing 400044, Peoples R China 2.CPI Yuanda Environm Protect Engn Co Ltd, Yuanda Grp, Chongqing 01122, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Xu, Guojing,Li, Dongwei,Jiao, Binquan,et al. Biomineralization of a calcifying ureolytic bacterium Microbacterium sp GM-1[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2017,25:21-27.
APA	Xu, Guojing.,Li, Dongwei.,Jiao, Binquan.,Li, Dou.,Yin, Yajie.,...&Li, Shan.(2017).Biomineralization of a calcifying ureolytic bacterium Microbacterium sp GM-1.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,25,21-27.
MLA	Xu, Guojing,et al."Biomineralization of a calcifying ureolytic bacterium Microbacterium sp GM-1".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 25(2017):21-27.