Deletion of mstna and mstnb impairs the immune system and affects growth performance in zebrafish

doi:10.1016/j.fsi.2017.11.040

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 环境与健康研究中心

	Deletion of mstna and mstnb impairs the immune system and affects growth performance in zebrafish
	Wang, Chao 1,2; Chen, Yan-Ling2 ; Bian, Wan-Ping2 ; Xie, Shao-Lin 1,2; Qi, Ge-Le 2; Liu, Li 2; Strauss, Phyllis R.3; Zou, Ji-Xing 1; Pei, De-Sheng2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018
卷号	72 页码:572-580
关键词	mstna mstnb Innate immune NF-kappa B Growth CRISPR/Cas9
ISSN号	1050-4648
DOI	10.1016/j.fsi.2017.11.040
英文摘要	Myostatin (Mstn) is a negative regulator of muscle development in vertebrates. Although its function in muscle growth has been well studied in mammals and fish, it remains unclear whether or how mstn functions in the immune system. In this study, mstna(-/-) and mstnb(-/-) homozygous zebrafish were firstly generated using CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9). Deletion of mstnb but not mstna enhanced growth performance. Although survival rates under normal conditions were slightly decreased in both strains, mortality after dexamethasone-induced stress was increased by 30%. Furthermore, transcriptional levels of several critical immune-related genes were decreased, and the ability to withstand exposure to pathogenic E. tarda was decreased, compared with that of controls. In mstnb but not mstna(-/-) zebrafish, expression of NF-kappa B subunits and several pro-inflammatory cytokines failed to respond to E. tarda exposure except nfkb1, c-rel and tnf alpha. Taken together, these results indicate that mstnb but not mstna plays a key role in zebrafish muscle growth. While each paralogue contributes to the response to bacterial insult, mstnb affects the immune system through activation of the NF-kappa B pathway, and mstna is likely to act upstream of NF-kappa B at some as yet unidentified target.
资助项目	Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20004] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20002] ; Hundred Talents Program of Chinese Academy of Sciences
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000423644600066
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/6244]
专题	环境与健康研究中心
通讯作者	Zou, Ji-Xing; Pei, De-Sheng
作者单位	1.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 3.Northeastern Univ, Coll Sci, Dept Biol, Boston, MA 02115 USA
推荐引用方式 GB/T 7714	Wang, Chao,Chen, Yan-Ling,Bian, Wan-Ping,et al. Deletion of mstna and mstnb impairs the immune system and affects growth performance in zebrafish[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:572-580.
APA	Wang, Chao.,Chen, Yan-Ling.,Bian, Wan-Ping.,Xie, Shao-Lin.,Qi, Ge-Le.,...&Pei, De-Sheng.(2018).Deletion of mstna and mstnb impairs the immune system and affects growth performance in zebrafish.FISH & SHELLFISH IMMUNOLOGY,72,572-580.
MLA	Wang, Chao,et al."Deletion of mstna and mstnb impairs the immune system and affects growth performance in zebrafish".FISH & SHELLFISH IMMUNOLOGY 72(2018):572-580.