Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy

doi:10.1364/BOE.8.004427

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 太赫兹技术研究中心

	Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy
	Zhao, Xiang 1; Zhang, Mingkun2 ; Wei, Dongshan 2; Wang, Yunxia 1; Yan, Shihan2 ; Liu, Mengwan 2; Yang, Xiang 1; Yang, Ke 1; Cui, Hong-Liang2 ; Fu, Weiling 1
刊名	BIOMEDICAL OPTICS EXPRESS
	2017-10-01
卷号	8 期号:10 页码:4427-4437
ISSN号	2156-7085
DOI	10.1364/BOE.8.004427
通讯作者	Cui, HL (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China.
英文摘要	The aptamer and target molecule binding reaction has been widely applied for construction of aptasensors, most of which are labeled methods. In contrast, terahertz technology proves to be a label-free sensing tool for biomedical applications. We utilize terahertz absorption spectroscopy and molecular dynamics simulation to investigate the variation of binding-induced collective vibration of hydrogen bond network in a mixed solution of MUC1 peptide and anti-MUC1 aptamer. The results show that binding-induced alterations of hydrogen bond numbers could be sensitively reflected by the variation of terahertz absorption coefficients of the mixed solution in a customized fluidic chip. The minimal detectable concentration is determined as 1 pmol/mu L, which is approximately equal to the optimal immobilized concentration of aptasensors. (C) 2017 Optical Society of America
资助项目	National Basic Research Program of China (Program 973)[2015CB755400] ; National Natural Science Foundation of China (NSFC)[81430054] ; National Natural Science Foundation of China (NSFC)[81370049] ; National Natural Science Foundation of China (NSFC)[61605206] ; National Natural Science Foundation of China (NSFC)[31400625]
WOS研究方向	Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	OPTICAL SOC AMER
WOS记录号	WOS:000412052000010
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/144]
专题	太赫兹技术研究中心
通讯作者	Cui, Hong-Liang
作者单位	1.Third Mil Med Univ, Southwest Hosp, Dept Lab Med, Chongqing 400038, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xiang,Zhang, Mingkun,Wei, Dongshan,et al. Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy[J]. BIOMEDICAL OPTICS EXPRESS,2017,8(10):4427-4437.
APA	Zhao, Xiang.,Zhang, Mingkun.,Wei, Dongshan.,Wang, Yunxia.,Yan, Shihan.,...&Fu, Weiling.(2017).Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy.BIOMEDICAL OPTICS EXPRESS,8(10),4427-4437.
MLA	Zhao, Xiang,et al."Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy".BIOMEDICAL OPTICS EXPRESS 8.10(2017):4427-4437.