Fuzzy logic sensing of G-quadruplex DNA and its cleavage reagents based on reduced graphene oxide | |
Huang, Wei Tao1; Zhang, Jian Rong1; Xie, Wan Yi2; Shi, Yan1; Luo, Hong Qun1; Li, Nian Bing1 | |
刊名 | BIOSENSORS & BIOELECTRONICS
![]() |
2014-07-15 | |
卷号 | 57页码:117-124 |
关键词 | Free Radical Fuzzy Logic Graphene G-quadruplex Sensor |
ISSN号 | 0956-5663 |
DOI | 10.1016/j.bios.2014.01.055 |
英文摘要 | Herein, by combining the merits of nanotechnology and fuzzy logic theory, we develop a simple, labelfree, and general strategy based on an organic dye-graphene hybrid system for fluorescence intelligent sensing of G-quadruplexes (G4) formation, hydroxyl radical (HO center dot),and Fe2+ in vitro. By exploiting acridine orange (AO) dyes-graphene as a nanofilter and nanoswitch and the ability of graphene to interact with DNA with different structures, our approach can efficiently distinguish, quantitatively detect target analytes. In vitro assays with G4DNA demonstrated increases in fluorescence intensity of the AO-rGO system with a linear range of 16-338 nM and a detection limit as low as 2.0 nM. The requenched fluorescence of the G4TBA-AO-rGO system has a non-linear response to Fenton reagent. But this requenching reduces the fluorescence intensity in a manner proportional to the logarithm to the base 10 of the concentration of Fenton reagent in the range of 0.1-100 mu M and 100-2000 mu M, respectively. Furthermore, we develop a novel and intelligent sensing method based on fuzzy logic which mimics human reasoning, solves complex and non-linear problems, and transforms the numerical output into the language description output for potential application in biochemical systems, environmental monitoring systems, and molecular-level fuzzy logic computing system. (C) 2014 Elsevier B.V. All rights reserved. |
资助项目 | National Natural Science Foundation of China[20975083] ; National Natural Science Foundation of China[21273174] ; Municipal Science Foundation of Chongqing City[CSTC-2013jjB00002] ; Fundamental Research Funds for the Central Universities[XDJK2013D006] ; Innovation and Technology Foundation of Southwest University for Excellent Doctoral Student[kb2011010] |
WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
语种 | 英语 |
出版者 | ELSEVIER ADVANCED TECHNOLOGY |
WOS记录号 | WOS:000334086100019 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.138/handle/2HOD01W0/1217] ![]() |
专题 | 中国科学院重庆绿色智能技术研究院 |
作者单位 | 1.Southwest Univ, Sch Chem & Chem Engn, Minist Educ, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Chongqing 400715, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China |
推荐引用方式 GB/T 7714 | Huang, Wei Tao,Zhang, Jian Rong,Xie, Wan Yi,et al. Fuzzy logic sensing of G-quadruplex DNA and its cleavage reagents based on reduced graphene oxide[J]. BIOSENSORS & BIOELECTRONICS,2014,57:117-124. |
APA | Huang, Wei Tao,Zhang, Jian Rong,Xie, Wan Yi,Shi, Yan,Luo, Hong Qun,&Li, Nian Bing.(2014).Fuzzy logic sensing of G-quadruplex DNA and its cleavage reagents based on reduced graphene oxide.BIOSENSORS & BIOELECTRONICS,57,117-124. |
MLA | Huang, Wei Tao,et al."Fuzzy logic sensing of G-quadruplex DNA and its cleavage reagents based on reduced graphene oxide".BIOSENSORS & BIOELECTRONICS 57(2014):117-124. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论