Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis

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	Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis
通讯作者	Shen, JH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, 300 Fenglin Rd, Shanghai 200032, Peoples R China.,jhshen@sibs.ac.cn
学科主题	Biotechnology & Applied Microbiology ; Microbiology
关键词	16s Ribosomal-rna Bacterioplankton Community Composition Cyanobacterial Blooms Recent Eutrophication Microbial Community Diversity China Pcr Dynamics Plankton
出处	JOURNAL OF APPLIED MICROBIOLOGY
ISSN号	1364-5072
	2009
卷号	106 期号:3 页码:1022-1032
产权排序	[Liu, F. H.; Lin, G. H.; Zhang, J. S.; Zhao, G. P.; Zhou, Z. H.; Shen, J. H.] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China; [Liu, F. H.] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Gao, G.; Qin, B. Q.] Chinese Acad Sci, Nanjing Inst Geog & Limnol, Nanjing, Peoples R China
资助机构	Taihu Laboratory for Lake Ecosystem Research (TLLER); Chinese Academy of Sciences
英文摘要	To explore the association of microbial community structure with the development of eutrophication in a large shallow freshwater lake, Lake Taihu. The bacterial and archaeal assemblages in sediments of different lake areas were analysed using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA fragments. The bacterial DGGE profiles showed that eutrophied sites, grass-bottom areas and relatively clean sites with a eutrophic (albeit dredged) site are three respective clusters. Fifty-one dominant bacterial DGGE bands were detected and 92 corresponding clones were sequenced, most of which were affiliated with bacterial phylotypes commonly found in freshwater ecosystems. Actinobacteria were detected in the centre of the lake and not at eutrophied sites whereas the opposite was found with respect to Verrucomicrobiales. Twenty-five dominant archaeal DGGE bands were detected and 31 corresponding clones were sequenced, most of which were affiliated with freshwater archaeal phylotypes. The bacterial community structures in the sediments of different areas with similar water quality and situation tend to be similar in Taihu Lake. This study may expand our knowledge on the relationship between the overall microbial assemblages and the development of eutrophication in the shallow freshwater lake.
语种	英语
内容类型	共享期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6323]
专题	烟台海岸带研究所_科研共享资源
推荐引用方式 GB/T 7714	. Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis. 2009.