Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte)

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 滨海湿地实验室

	Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte)
	Chen, Peng 1,4; Shao, HongBo 1,2; Xu, Di 3
刊名	AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
	2011-09-16
卷号	5 期号:18 页码:2590-2595
关键词	Gracilaria Lemaneiformis Suppression Subtractive Hybridization (Ssh) Sex Determination Gametophyte
ISSN号	1996-0808
通讯作者	Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Environm Proc, CAS Shandong Prov, Yantai 264003, Peoples R China. shaohongbochu@126.com
产权排序	[Chen, Peng; Shao, HongBo] Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Environm Proc, CAS Shandong Prov, Yantai 264003, Peoples R China; [Shao, HongBo] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China; [Xu, Di] Ocean Univ China, Coll Marine Life Sci, Qingdao 266002, Peoples R China; [Chen, Peng] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	In this study, forward and reverse suppression subtractive hybridization (SSH) libraries were constructed between female and male gametophyte of Gracilaria lemaneiformis to isolate genes differentially expressed between gametophytes. Dot-blots were performed to screen 384 colonies randomly selected from each subtracted libraries. Partial gene (designated as GMF-01) which is female gametophyte-specific was selected to isolate its cDNA full length sequences and to be characterized. Then we got its cDNA full length sequences using SMART-RACE technic. Sequence analysis showed that the open reading frame (ORF) of GMF-01 is 1002 bp long with a GC content of 47.7%, encoding 333 amino acids. GMF-01 does not have significant match in the databases when examined on NCBI website. Amino acid sequence analysis showed that the theoretical pI of this translated protein is 7.92, while the instability index is computed to be 43.61, which has classified the protein as unstable. Results of prediction showed 45.05% of its secondary structure has random coil and sub-cellular location prediction indicated that it is probably a extracellular protein.; In this study, forward and reverse suppression subtractive hybridization (SSH) libraries were constructed between female and male gametophyte of Gracilaria lemaneiformis to isolate genes differentially expressed between gametophytes. Dot-blots were performed to screen 384 colonies randomly selected from each subtracted libraries. Partial gene (designated as GMF-01) which is female gametophyte-specific was selected to isolate its cDNA full length sequences and to be characterized. Then we got its cDNA full length sequences using SMART-RACE technic. Sequence analysis showed that the open reading frame (ORF) of GMF-01 is 1002 bp long with a GC content of 47.7%, encoding 333 amino acids. GMF-01 does not have significant match in the databases when examined on NCBI website. Amino acid sequence analysis showed that the theoretical pI of this translated protein is 7.92, while the instability index is computed to be 43.61, which has classified the protein as unstable. Results of prediction showed 45.05% of its secondary structure has random coil and sub-cellular location prediction indicated that it is probably a extracellular protein.
学科主题	Microbiology
WOS关键词	SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; RED ALGA ; IDENTIFICATION ; DIFFERENTIATION ; EXPRESSION ; LIBRARIES
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000298919300006
资助机构	National Scientific Foundations of China[40606034]
公开日期	2012-03-06
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5307]
专题	烟台海岸带研究所_滨海湿地实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Environm Proc, CAS Shandong Prov, Yantai 264003, Peoples R China 2.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China 3.Ocean Univ China, Coll Marine Life Sci, Qingdao 266002, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Peng,Shao, HongBo,Xu, Di. Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte)[J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,2011,5(18):2590-2595.
APA	Chen, Peng,Shao, HongBo,&Xu, Di.(2011).Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte).AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,5(18),2590-2595.
MLA	Chen, Peng,et al."Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte)".AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 5.18(2011):2590-2595.