Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 污染过程与控制实验室

	Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum
	Zhao, Jianmin1 ; Li, Chenghua 1,2; Chen, Aiqin 1; Li, Lingyun 3; Su, Xiurong 2; Li, Taiwu 2
刊名	PLOS ONE
	2010-10-20
卷号	5 期号:10 页码:e13480-
关键词	Oyster Crassostrea-gigas Antimicrobial Peptides Expression Gene Shrimp Family Penaeidins Proteins Mussels Crab
ISSN号	1932-6203
通讯作者	Zhao, JM, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
产权排序	[Zhao, Jianmin; Li, Chenghua; Chen, Aiqin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Li, Chenghua; Su, Xiurong; Li, Taiwu] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo, Peoples R China; [Li, Lingyun] NE Agr Univ, Coll Anim Sci & Technol, Harbin, Peoples R China
文献子类	Article
英文摘要	Antimicrobial peptides (AMPs) are important mediators of the primary defense mechanism against microbial invasion. In the present study, a big defensin was identified from Venerupis philippinarum haemocytes (denoted as VpBD) by RACE and EST approaches. The VpBD cDNA contained an open reading frame (ORF) of 285 bp encoding a polypeptide of 94 amino acids. The deduce amino acid sequence of VpBD shared the common features of big defensin including disulfide array organization and helix structure, indicating that VpBD should be a new member of the big defensin family. The mRNA transcript of VpBD was up-regulated significantly during the first 24 hr after Vibrio anguillarum challenge, which was 7.4-fold increase compared to that of the control group. Then the expression decreased gradually from 24 hr to 96 hr, and the lowest expression level was detected at 96 hr post-infection, which was still 3.9-fold higher than that of control. The mature peptide of VpBD was recombined in Escherichia coli and purified for minimum inhibitory concentration (MIC) determination. The rVpBD displayed broad-spectrum inhibitory activity towards all tested bacteria with the highest activity against Staphyloccocus aureus and Pseudomonas putida. These results indicated that VpBD was involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria.; Antimicrobial peptides (AMPs) are important mediators of the primary defense mechanism against microbial invasion. In the present study, a big defensin was identified from Venerupis philippinarum haemocytes (denoted as VpBD) by RACE and EST approaches. The VpBD cDNA contained an open reading frame (ORF) of 285 bp encoding a polypeptide of 94 amino acids. The deduce amino acid sequence of VpBD shared the common features of big defensin including disulfide array organization and helix structure, indicating that VpBD should be a new member of the big defensin family. The mRNA transcript of VpBD was up-regulated significantly during the first 24 hr after Vibrio anguillarum challenge, which was 7.4-fold increase compared to that of the control group. Then the expression decreased gradually from 24 hr to 96 hr, and the lowest expression level was detected at 96 hr post-infection, which was still 3.9-fold higher than that of control. The mature peptide of VpBD was recombined in Escherichia coli and purified for minimum inhibitory concentration (MIC) determination. The rVpBD displayed broad-spectrum inhibitory activity towards all tested bacteria with the highest activity against Staphyloccocus aureus and Pseudomonas putida. These results indicated that VpBD was involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria.
学科主题	Biology ; Multidisciplinary Sciences
URL标识	查看原文
WOS关键词	OYSTER CRASSOSTREA-GIGAS ; ANTIMICROBIAL PEPTIDES ; EXPRESSION ; GENE ; SHRIMP ; FAMILY ; PENAEIDINS ; PROTEINS ; MUSSELS ; CRAB
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000283216400009
资助机构	Chinese Academy of Sciences [kzcx2-yw-225]; NSFC [30901115]
公开日期	2011-07-14
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/3796]
专题	烟台海岸带研究所_污染过程与控制实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo, Peoples R China 3.NE Agr Univ, Coll Anim Sci & Technol, Harbin, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jianmin,Li, Chenghua,Chen, Aiqin,et al. Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum[J]. PLOS ONE,2010,5(10):e13480-.
APA	Zhao, Jianmin,Li, Chenghua,Chen, Aiqin,Li, Lingyun,Su, Xiurong,&Li, Taiwu.(2010).Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum.PLOS ONE,5(10),e13480-.
MLA	Zhao, Jianmin,et al."Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum".PLOS ONE 5.10(2010):e13480-.