Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models | |
Wang, Rui1,2; Han, Xiaoyue1,2; You, Jinmao1,2; Yu, Fabiao1,2; Chen, Lingxin1,2 | |
刊名 | ANALYTICAL CHEMISTRY |
2018-03-20 | |
卷号 | 90期号:6页码:4054-4061 |
ISSN号 | 0003-2700 |
DOI | 10.1021/acs.analchem.7b05297 |
文献子类 | Article |
英文摘要 | As new biomarkers, monoamine oxidases (MAOs) play important roles in maintaining the homeostasis of biogenic amines via catalyzing the oxidation of biogenic amines to corresponding aldehydes with the generation of reactive oxygen species (ROS). MAOs have two isoforms, MAO-A and MAO-B. MAO-A is considered to be a major factor of neuropsychiatric and depressive disorders. However, MAO-B is thought to be involved in several neurodegenerative diseases. Therefore, to explore their distinct roles in different diseases, the selective detection of MAOs is essential. Herein, two new types of near-infrared (NIR) fluorescent probes, MitoCy-NH2 and MitoHCy-NH2, are provided for synergistic imaging of MAO-B and its contribution to oxidative stress in cells and in mice aging models. These probes are composed of three moieties: heptamethine cyanine as fluorophore, propanamide as recognition group, and triphenylphosphonium cation as mitochondrial targeting group. The amine oxidation and beta-elimination reaction can lead to obvious fluorescence increase and color changes from green to blue. The probe MitoHCy-NH2 can be used to synergistically detect MAO-B and its contribution to oxidative stress in the replicative senescence model. And the probe MitoCy-NH2 can offer ratiometric near-infrared fluorescence for the selective detection of MAO-B in the H2O2-induced cell aging model and in mice aging models. The results reveal that there are different MAO-B levels in different ages of mice models. MitoCy-NH2 also can evaluate therapeutic effects of pargyline and selegiline in mice models. The desirable analytical behaviors of our probes make them useful chemical tools for the selective detection of MAO-B and its contribution to oxidative stress in biosystems. |
WOS关键词 | LIVING CELLS ; SUPEROXIDE ANION ; FLUOROGENIC PROBE ; REACTIVE OXYGEN ; IN-VIVO ; MAO-B ; DESIGN ; VISUALIZATION ; PLATFORMS ; CATIONS |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000428219600058 |
资助机构 | National Nature Science Foundation of China(21775162, 21405172, 41776110, 21575159) ; program of Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for EcoEnvironmental Sciences, CAS(KF2016-22) ; Instrument Developing Project of the Chinese Academy of Sciences(YZ201662) |
内容类型 | 期刊论文 |
源URL | [http://ir.yic.ac.cn/handle/133337/24031] |
专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
通讯作者 | Yu, Fabiao; Chen, Lingxin |
作者单位 | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn Technol Shandong Pr, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Rui,Han, Xiaoyue,You, Jinmao,et al. Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models[J]. ANALYTICAL CHEMISTRY,2018,90(6):4054-4061. |
APA | Wang, Rui,Han, Xiaoyue,You, Jinmao,Yu, Fabiao,&Chen, Lingxin.(2018).Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models.ANALYTICAL CHEMISTRY,90(6),4054-4061. |
MLA | Wang, Rui,et al."Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models".ANALYTICAL CHEMISTRY 90.6(2018):4054-4061. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论