A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress

doi:10.1039/c7sc02888a

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress
	Han, Xiaoyue 1,2; Song, Xinyu 1; Yu, Fabiao 1; Chen, Lingxin1 ; Yu, Fabiao(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
刊名	CHEMICAL SCIENCE
	2017-10-01
卷号	8 期号:10 页码:6991-7002
ISSN号	2041-6520
DOI	10.1039/c7sc02888a
产权排序	第1完成单位
文献子类	Article
英文摘要	Hypothermia and hyperthermia are cell stressed states resulting from environmental temperature changes, which can abnormally decrease intracellular glutathione (GSH) concentrations and induce apoptosis. As the most abundant intracellular non-protein biothiol, GSH can protect cells from apoptosis. Considering the important roles of GSH in the anti-apoptotic process in cells and in vivo, we strive to develop a powerful chemical tool for the direct detection of GSH concentration changes under temperature stress. Herein, we report a ratiometric fluorescent probe (CyO-Dise) based on a selenium-sulfur exchange reaction for the qualitative and quantitative detection of GSH concentration fluctuations in cells and in vivo. The probe has been successfully used to assess the changes of GSH levels in HepG2 and HL-7702 cells using the stimulations of hypothermia and hyperthermia. In terms of the anti-apoptotic effect of GSH under hypothermic and hyperthermic conditions, human normal liver HL-7702 cells have stronger abilities to fight against temperature stress than human liver carcinoma HepG2 cells. Hypothermia and hyperthermia can also improve the drug resistance of cis-dichlorodiamineplatinum(II) (DDP)-resistant HepG2/DDP cells. The CyO-Dise probe has been employed to image GSH concentration changes in HepG2 and HepG2/DDP xenografts on nude mice. With the adjuvant therapy effects of hypothermia and hyperthermia, the chemotherapy drug DDP exhibits good ability for the treatment of HepG2 and HepG2/DDP xenografts. The above applications make our probe a potential new candidate for the accurate diagnosis of cancer and efficacy evaluation of treatment.
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WOS关键词	HIGHLY SELECTIVE DETECTION ; SE-N BOND ; GENE-EXPRESSION ; LIVING CELLS ; DESIGN STRATEGIES ; REACTIVE OXYGEN ; IN-VIVO ; GLUTATHIONE ; THIOLS ; INDUCTION
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000411730500032
资助机构	National Nature Science Foundation of China(21405172, 21775162, 21575159) ; Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS(KF2016-22) ; Research Center for Eco-Environmental Sciences, CAS(KF2016-22)
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/23454]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Yu, Fabiao; Chen, Lingxin; Yu, Fabiao(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,et al. A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress[J]. CHEMICAL SCIENCE,2017,8(10):6991-7002.
APA	Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,Chen, Lingxin,Yu, Fabiao,&Chen, Lingxin.(2017).A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress.CHEMICAL SCIENCE,8(10),6991-7002.
MLA	Han, Xiaoyue,et al."A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress".CHEMICAL SCIENCE 8.10(2017):6991-7002.