Biosynthesis of a stable allophycocyanin beta subunit in metabolically engineered Escherichia coli
Chen, Huaxin1; Lin, Hanzhi1,2; Li, Fuchao1; Jiang, Peng1; Qin, Song3
刊名JOURNAL OF BIOSCIENCE AND BIOENGINEERING
2013-05-01
卷号115期号:5页码:485-489
关键词Allophycocyanin Biosynthesis Escherichia Coli Fluorescence Thermostability
ISSN号1389-1723
通讯作者Jiang, P (reprint author), Room 335,Biol Bldg,7 Nanhai Rd, Qingdao 266071, Peoples R China. jiangpeng@qdio.ac.cn
产权排序[Chen, Huaxin; Lin, Hanzhi; Li, Fuchao; Jiang, Peng] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China; [Lin, Hanzhi] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
文献子类Article
英文摘要Allophycocyanin (APC) is widely used as a fluorescent tag for fluorescence detection techniques. In this study, the alpha pcB gene from a thermophilic cyanobacterium strain was cloned and ligated into an expression vector to construct a pathway for the biosynthesis of an allophycocyanin beta subunit (holo-apcBT) in Escherichia coli. Isopropyl beta-D-1-thiogalactopyranoside induction successfully reconstituted holo-apcBT in E. coli. The recombinant holo-apcB showed spectroscopic properties similar to native APC. The stability and spectroscopic properties of this protein were then compared with another recombinant allophycocyanin beta subunit (holo-apcBM) whose apcB gene was cloned from mesophilic cyanobacterium. At high temperatures and during the course of storage, holo-apcBT was significantly more stable than holo-apcBM. In addition, holo-apcBT had an unexpectedly higher extinction coefficient and fluorescence quantum yield than holo-apcBM, suggesting that holo-apcBT would be a promising fluorescent tag and serve as a substitute for native APC. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.; Allophycocyanin (APC) is widely used as a fluorescent tag for fluorescence detection techniques. In this study, the alpha pcB gene from a thermophilic cyanobacterium strain was cloned and ligated into an expression vector to construct a pathway for the biosynthesis of an allophycocyanin beta subunit (holo-apcBT) in Escherichia coli. Isopropyl beta-D-1-thiogalactopyranoside induction successfully reconstituted holo-apcBT in E. coli. The recombinant holo-apcB showed spectroscopic properties similar to native APC. The stability and spectroscopic properties of this protein were then compared with another recombinant allophycocyanin beta subunit (holo-apcBM) whose apcB gene was cloned from mesophilic cyanobacterium. At high temperatures and during the course of storage, holo-apcBT was significantly more stable than holo-apcBM. In addition, holo-apcBT had an unexpectedly higher extinction coefficient and fluorescence quantum yield than holo-apcBM, suggesting that holo-apcBT would be a promising fluorescent tag and serve as a substitute for native APC. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.
学科主题Biotechnology & Applied Microbiology ; Food Science & Technology
URL标识查看原文
WOS关键词SYNECHOCOCCUS SP PCC-7002 ; C-PHYCOCYANIN ; CYANOBACTERIAL PHYCOBILIPROTEINS ; CPCS-I ; PROTEIN ; LYASE ; BIOGENESIS ; STABILITY ; PEPTIDES ; TRIMER
WOS研究方向Biotechnology & Applied Microbiology ; Food Science & Technology
语种英语
WOS记录号WOS:000320208600004
资助机构Natural Science Foundation of Shandong Province [2009ZRB02542]; Foundation of the Key Laboratory of Marine Bioactive Substance and Modern Analytical Techniques, State Oceanic Administration [MBSMAT-2010-03]; Nonprofit Research Project for the State Oceanic Administration [200905021-3, 201205027-2]
公开日期2013-08-14
内容类型期刊论文
源URL[http://ir.yic.ac.cn/handle/133337/6413]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714
Chen, Huaxin,Lin, Hanzhi,Li, Fuchao,et al. Biosynthesis of a stable allophycocyanin beta subunit in metabolically engineered Escherichia coli[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2013,115(5):485-489.
APA Chen, Huaxin,Lin, Hanzhi,Li, Fuchao,Jiang, Peng,&Qin, Song.(2013).Biosynthesis of a stable allophycocyanin beta subunit in metabolically engineered Escherichia coli.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,115(5),485-489.
MLA Chen, Huaxin,et al."Biosynthesis of a stable allophycocyanin beta subunit in metabolically engineered Escherichia coli".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 115.5(2013):485-489.
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