Molecular Evolution of Lycopene Cyclases Involved in the Formation of Carotenoids in Eukaryotic Algae

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	Molecular Evolution of Lycopene Cyclases Involved in the Formation of Carotenoids in Eukaryotic Algae
	Cui, Hongli 1,2; Wang, Yinchu1,2 ; Qin, Song1
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2011-12-01
卷号	29 期号:4 页码:1013-1020
关键词	Carotenoid Biosynthesis Lycopene Cyclase Molecular Evolution Coevolution Algae
ISSN号	0735-9640
通讯作者	Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
产权排序	[Cui, Hongli; Wang, Yinchu; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Cui, Hongli; Wang, Yinchu] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	Carotenoids play crucial roles in structure and function of the photosynthetic apparatus of bacteria, algae, and higher plants. The formation of carotenoids from lycopene is catalyzed by the enzyme lycopene cyclase (LCY), which is structurally and functionally conserved in all organisms. A comparative genomic analysis regarding the LCY revealed that the higher plant (Arabidopsis thaliana) and the green alga (Ostreococcus sp. RCC809, Ostreococcus tauri, Ostreococcus lucimarinus, Micromonas sp. RCC299, Micromonas pusiua, Chlorella vulgaris, Volvox carteri, and Coccomyxa sp. C-169) possess two different LCY (beta- and epsilon-type). This indicated that an ancient gene duplication event must have occurred, which produced two classes of LCY in algae. However, some other green alga retained only one class of LCY, such as Haematococcus pluvialis (beta), Dunaliella salina (beta), Chlamydomonas reinhardtii (epsilon), and Chlorella sp. NC64A (epsilon), and the other gene copy was lost in these species. Furthermore, the similar LCY lost occurred in red alga (Cyanidioschyzon merolae) and Heterokontophyta (Phaeodactylum tricornutum and Thalassiosira pseudonana), which possess only the LCYB. In addition, the protein sequence of LCYB is highly similar to capsanthin-capsorubin synthase (CCS), which is another carotenogenic enzyme of plants. As a result, it is proposed that the CCS evolved from a duplicated LCYB. The discovery of two classes of LCY families in some algae suggests that carotenoid biosynthesis is differentially regulated in response to development and environmental stress in these algae, like members of LCY families are differentially regulated during development or stress in some higher plants.; Carotenoids play crucial roles in structure and function of the photosynthetic apparatus of bacteria, algae, and higher plants. The formation of carotenoids from lycopene is catalyzed by the enzyme lycopene cyclase (LCY), which is structurally and functionally conserved in all organisms. A comparative genomic analysis regarding the LCY revealed that the higher plant (Arabidopsis thaliana) and the green alga (Ostreococcus sp. RCC809, Ostreococcus tauri, Ostreococcus lucimarinus, Micromonas sp. RCC299, Micromonas pusiua, Chlorella vulgaris, Volvox carteri, and Coccomyxa sp. C-169) possess two different LCY (beta- and epsilon-type). This indicated that an ancient gene duplication event must have occurred, which produced two classes of LCY in algae. However, some other green alga retained only one class of LCY, such as Haematococcus pluvialis (beta), Dunaliella salina (beta), Chlamydomonas reinhardtii (epsilon), and Chlorella sp. NC64A (epsilon), and the other gene copy was lost in these species. Furthermore, the similar LCY lost occurred in red alga (Cyanidioschyzon merolae) and Heterokontophyta (Phaeodactylum tricornutum and Thalassiosira pseudonana), which possess only the LCYB. In addition, the protein sequence of LCYB is highly similar to capsanthin-capsorubin synthase (CCS), which is another carotenogenic enzyme of plants. As a result, it is proposed that the CCS evolved from a duplicated LCYB. The discovery of two classes of LCY families in some algae suggests that carotenoid biosynthesis is differentially regulated in response to development and environmental stress in these algae, like members of LCY families are differentially regulated during development or stress in some higher plants.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
URL标识	查看原文
WOS关键词	FUNCTIONAL-ANALYSIS ; GENE-TRANSFER ; ISOPRENOID BIOSYNTHESIS ; PHYTOENE SYNTHASE ; CRT GENES ; PLANTS ; PATHWAY ; PHYLOGENIES ; ENZYMES ; CLONING
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
WOS记录号	WOS:000296068700027
资助机构	National Natural Science Foundation of China[40876082]; International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area
公开日期	2012-03-06
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5355]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cui, Hongli,Wang, Yinchu,Qin, Song. Molecular Evolution of Lycopene Cyclases Involved in the Formation of Carotenoids in Eukaryotic Algae[J]. PLANT MOLECULAR BIOLOGY REPORTER,2011,29(4):1013-1020.
APA	Cui, Hongli,Wang, Yinchu,&Qin, Song.(2011).Molecular Evolution of Lycopene Cyclases Involved in the Formation of Carotenoids in Eukaryotic Algae.PLANT MOLECULAR BIOLOGY REPORTER,29(4),1013-1020.
MLA	Cui, Hongli,et al."Molecular Evolution of Lycopene Cyclases Involved in the Formation of Carotenoids in Eukaryotic Algae".PLANT MOLECULAR BIOLOGY REPORTER 29.4(2011):1013-1020.