Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics

doi:10.1016/j.aquatox.2017.09.010

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院烟台海岸带研究所知识产出

	Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics
	Song, Qinqin 1,2,3; Zhou, Hailong 1,2; Han, Qian 1,2; Diao, Xiaoping 1,2
刊名	AQUATIC TOXICOLOGY
	2017-11-01
卷号	192 页码:48-57
关键词	Benzo(a)Pyrene Ddt P. Viridis Hepatopancreas Itraq Metabolomics
ISSN号	0166-445X
DOI	10.1016/j.aquatox.2017.09.010
文献子类	Article
英文摘要	Dichlorodiphenyltrichloroethane (DDT) and benzo(a)pyrene (BaP) are environmental estrogens (EEs) that are ubiquitous in the marine environment. In the present study, we integrated isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic and nuclear magnetic resonance (NMR)-based metabolomic approaches to explore the toxic responses of green mussel hepatopancreas exposed to DDT (10 mu g/L), BaP (10 mu g/L) and their mixture. The metabolic responses indicated that BaP primarily disturbed energy metabolism and osmotic regulation in the hepatopancreas of the male green mussel P. viridis. Both DDT and the mixture of DDT and BaP perturbed the energy metabolism and osmotic regulation in P. viridis. The proteomic responses revealed that BaP affected the proteins involved in energy metabolism, material transformation, cytoskeleton, stress responses, reproduction and development in green mussels. DDT exposure could change the proteins involved in primary metabolism, stress responses, cytoskeleton and signal transduction. However, the mixture of DDT and BaP altered proteins associated with material and energy metabolism, stress responses, signal transduction, reproduction and development, cytoskeleton and apoptosis. This study showed that iTRAQ-based proteomic and NMR-based metabolomic approaches could effectively elucidate the essential molecular mechanism of disturbances in hepatopancreas function of green mussels exposed to environmental estrogens.
WOS关键词	MUSSEL MYTILUS-GALLOPROVINCIALIS ; CLAM RUDITAPES-PHILIPPINARUM ; EARTHWORM EISENIA-FOETIDA ; OYSTER CRASSOSTREA-GIGAS ; HEAT-SHOCK PROTEINS ; GENDER-SPECIFIC RESPONSES ; 2,2',4,4'-TETRABROMODIPHENYL ETHER ; PETROCHEMICAL CONTAMINATION ; H-1-NMR SPECTROSCOPY ; SALINITY ACCLIMATION
WOS研究方向	Marine & Freshwater Biology ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000415768700007
资助机构	National Natural Science Foundation of China(41161077, 31760164) ; Specialized Research Fund for the Midwest Program of Hainan University(ZXBJH-XK002)
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24046]
专题	烟台海岸带研究所_中科院烟台海岸带研究所知识产出
通讯作者	Zhou, Hailong
作者单位	1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 2.Hainan Univ, Inst Trop Agr & Forestry, 58 Renmin Rd, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Song, Qinqin,Zhou, Hailong,Han, Qian,et al. Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics[J]. AQUATIC TOXICOLOGY,2017,192:48-57.
APA	Song, Qinqin,Zhou, Hailong,Han, Qian,&Diao, Xiaoping.(2017).Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.AQUATIC TOXICOLOGY,192,48-57.
MLA	Song, Qinqin,et al."Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a) pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics".AQUATIC TOXICOLOGY 192(2017):48-57.