Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling

doi:10.1038/s41598-017-07740-5

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
	Ren, Cheng-Gang 1; Kong, Cun-Cui 1; Yan, Kun2 ; Zhang, Hua2 ; Luo, Yong-Ming2 ; Xie, Zhi-Hong 1; Xie, Zhi-Hong(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Biol & Utilizat Biol Resources Coastal Zo, Yantai 264003, Peoples R China)
刊名	SCIENTIFIC REPORTS
	2017-08-23
卷号	7
ISSN号	2045-2322
DOI	10.1038/s41598-017-07740-5
产权排序	第1完成单位 ; 第2完成单位
文献子类	Article
英文摘要	Sesbania cannabina, a multipurpose leguminous crop, is highly resistant to waterlogging stress. However, the scant genomic resources in the genus Sesbania have greatly hindered further exploration of the mechanisms underlying its waterlogging tolerance. Here, the genetic basis of flooding tolerance in S. cannabina was examined by transcriptome-wide gene expression changes using RNA-Seq in seedlings exposed to short-term (3 h) and long-term (27 h) waterlogging. After de-novo assembly, 213990 unigenes were identified, of which 145162 (79.6%) were annotated. Gene Ontology and pathway enrichment analyses revealed that the glycolysis and fermentation pathways were stimulated to produce ATP under hypoxic stress conditions. Energy-consuming biosynthetic processes were dramatically repressed by short and long term waterlogging, while amino acid metabolism was greatly induced to maintain ATP levels. The expression pattern of 10 unigenes involved in phenylpropanoid biosynthesis, glycolysis, and amino acid metabolism revealed by qRT-PCR confirmed the RNA-Seq data. The present study is a large-scale assessment of genomic resources of Sesbania and provides guidelines for probing the molecular mechanisms underlying S. cannabina waterlogging tolerance.
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WOS关键词	GENE-EXPRESSION RESPONSES ; RNA-SEQ DATA ; LOW-OXYGEN ; DROUGHT STRESS ; SUBMERGENCE TOLERANCE ; MAIZE SEEDLINGS ; ARABIDOPSIS ; LEAVES ; HYPOXIA ; RICE
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000408434500005
资助机构	One Hundred-Talent Plan of Chinese Academy of Sciences(Y529041011) ; National Natural Science Foundation of China(31601238, 31370108, 31570063) ; High-tech Industrialization Cooperation Funds of Jilin province ; Chinese Academy of Science ; Shandong Key Research and Development Program(2016CYJS05A01-1) ; Shandong Academy of Agricultural Sciences(+CXGC2016B10) ; Yantai Key Project of Research and Development Plan(2016ZH074)
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/23433]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Xie, Zhi-Hong; Xie, Zhi-Hong(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Biol & Utilizat Biol Resources Coastal Zo, Yantai 264003, Peoples R China)
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Biol & Utilizat Biol Resources Coastal Zo, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Ren, Cheng-Gang,Kong, Cun-Cui,Yan, Kun,et al. Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling[J]. SCIENTIFIC REPORTS,2017,7.
APA	Ren, Cheng-Gang.,Kong, Cun-Cui.,Yan, Kun.,Zhang, Hua.,Luo, Yong-Ming.,...&Xie, Zhi-Hong.(2017).Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling.SCIENTIFIC REPORTS,7.
MLA	Ren, Cheng-Gang,et al."Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling".SCIENTIFIC REPORTS 7(2017).